CHARACTERIZATION OF A T(10-11)(P13-14-Q14-21) IN THE MONOBLASTIC CELL-LINE U937

被引：9

作者：

SHIPLEY, J

WILLIAMS, S

OBYRNE, A

KEARNEY, L

JONES, T

YOUNG, B

DYER, M

CATOVSKY, D

SHEER, D

GUSTERSON, B

机构：

[1] ST BARTHOLOMEWS HOSP,HUMAN CYTOGENET LAB,LONDON,ENGLAND

[2] ST BARTHOLOMEWS HOSP,DEPT MED ONCOL,LONDON,ENGLAND

[3] ROYAL MARSDEN HOSP,IMPERIAL CANC RES FUND,LONDON SW3 6JJ,ENGLAND

[4] ROYAL MARSDEN HOSP,ACAD DEPT HAEMATOL & CYTOGENET,LONDON SW3 6JJ,ENGLAND

[5] INST CANC RES,LONDON SW3 6JB,ENGLAND

[6] JOHN RADCLIFFE HOSP,INST MOLEC MED,MRC,MOLEC HAEMATOL UNIT,OXFORD OX3 9DU,ENGLAND

来源：

GENES CHROMOSOMES & CANCER | 1995年 / 13卷 / 02期

关键词：

D O I：

10.1002/gcc.2870130211

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Previous analysis of the monoblastic cell line U937 has shown that several sublines contain a rearranged chromosome arm 11q. In order to determine the true nature of the rearrangement, fluorescence in situ hybridization (FISH) was carried out with various combinations of single copy anonymous markers, clones containing genes, a chromosome 10 paint, and an 11 centromere specific sequence. The rearrangement was deduced to be a reciprocal translocation between chromosomes 10 and 11 described as t(10;11)(p 13-14;9 14-21). The breakpoint on chromosome 11 is telomeric to the INT2 gene and the pHS 11 probe at 11 q 13, and centromeric to the marker D11S36 localized to 11q14.3-q22.1 and the MLL gene at 11q23. Similar translocations have been reported in various acute leukemias, principally of the monocytic lineage, and also in T-cell precursor acute lymphocytic leukemias. Further characterization of the genetic rearrangements in U937 may lead to the isolation of genes important in leukemogenesis and provide an in vitro system for their study. (C) 1995 Wiley-Liss, Inc.

引用

页码：138 / 142

页数：5

共 41 条

[1]

Alkema M, Weigant J, Raap A, Berns A, Lohuizen MV, Characterisation and chromosomal localization of the human proto‐oncogene BMI‐1, Hum Mol Genet, 2, pp. 1597-1603, (1993)

[2]

Berger R, Flandrin G, Bernheim A, Le-Coniat M, Vecchione D, Pacot A, Derre J, Daniel MT, Valensi F, Sigaux F, OchoaNoguera ME, Cytogenetic studies on 519 consecutive de novo acute nonlymphocytic leukemias, Cancer Genet Cytogenet, 29, pp. 9-21, (1987)

[3]

Brodeur G, Williams D, Kalwinsky D, Williams K, Dahl G, Cytogenetic features of acute nonlymphoblastic leukemia in 73 children and adolescents, Cancer Genet Cytogenet, 8, pp. 93-105, (1983)

[4]

Brookes S, Lamrnie GA, Schuuring E, De Boer C, Michalides R, Dickson C, Peters G, Amplified region of chromosome band 11q13 in breast and squamous cell carcinomas encompasses three CpG islands telomeric of FGF3, including the expressed gene EMSI, Genes Chromosom Cancer, 6, pp. 222-231, (1993)

[5]

Broustet A, Bernard P, Dachary D, David B, Marit G, Lacombe F, Issanchou A, Reiffers J, Acute eosinophilic leukaemia with a translocation (lop+

[6]

11q‐), Cancer Genet Cytogenet, 21, pp. 327-333, (1986)

[7]

Carter M, Kalwinsky D, Mirro J, Behm F, Head D, Huddleston T, Raimondi C, The t(10

[8]

11) (p14

[9]

q21) translocation in three children with acute myeloblastic leukemia, Leukemia, 5, pp. 561-565, (1991)

[10]

Fischoff S, Testa J, Schiffer C, Acute eosinophilic leukaemia with a (10

← 1 2 3 4 5 →