GAS-CHROMATOGRAPHY NEGATIVE CHEMICAL IONIZATION MASS-SPECTROMETRY OF INTACT GLUCURONIDES

被引：9

作者：

BROWN, SY ^{[1
]}

GARLAND, WA ^{[1
]}

FUKUDA, EK ^{[1
]}

机构：

[1] HOFFMANN LA ROCHE INC,DEPT DRUG METAB,NUTLEY,NJ 07110

来源：

BIOMEDICAL AND ENVIRONMENTAL MASS SPECTROMETRY | 1990年 / 19卷 / 01期

关键词：

D O I：

10.1002/bms.1200190105

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

Gas chromatography‐negative chemical ionization mass spectrometry (GC–NCIMS) has been applied to the analysis of O‐ and S‐glucuronides. The glucuronides were derivatized with pentafluorobenzyl (PFB) bromide and Tri‐Sil, and analyzed by GC–NCIMS. All glucuronides gave a large [M—PFB]− ion indicative of the molecular weight of the compound. This methodology has been successfully applied to the identification of a glucuronide of unknown structure. Copyright © 1990 John Wiley & Sons, Ltd.

引用

收藏

页码：32 / 36

页数：5

相关论文

共 14 条

[1] BILE-ACID GLUCURONIDES .3. CHEMICAL SYNTHESIS AND CHARACTERIZATION OF GLUCURONIC ACID COUPLED MONOHYDROXY, DIHYDROXY AND TRIHYDROXY BILE-ACIDS [J].

BACK, P ;

BOWEN, DV .

HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1976, 357 (02) :219-224

[2] N-NITROSOBUTYL-N-(4-HYDROXYBUTYL)AMINE GLUCURONIDE INVITRO SYNTHESIS, CHARACTERIZATION BY MASS-SPECTROMETRY AND DETERMINATION BY GAS-CHROMATOGRAPHY THERMAL-ENERGY ANALYSIS [J].

BOIGEGRAIN, RA ;

BONFANTI, M ;

FANELLI, R ;

AIROLDI, L .

JOURNAL OF CHROMATOGRAPHY, 1986, 383 (01) :172-178

[3]

BROWN SY, IN PRESS DRUG METAB

[4]

FENSELAU C, 1980, DRUG METAB DISPOS, V8, P274

[5]

GARTEIZ DA, 1985, LC MAG, V3, P334

[6] SENSITIVE DETERMINATION OF DEUTERATED AND NON-DEUTERATED PHENYLALANINE AND TYROSINE IN HUMAN-PLASMA BY COMBINED CAPILLARY GAS-CHROMATOGRAPHY - NEGATIVE-ION CHEMICAL IONIZATION MASS-SPECTROMETRY [J].

HAYASHI, T ;

MINATOGAWA, Y ;

KAMADA, S ;

SHIMAMURA, M ;

NARUSE, H ;

IIDA, Y .

JOURNAL OF CHROMATOGRAPHY, 1986, 380 (02) :239-245

[7] A MASS-SPECTROMETRIC APPROACH TO THE IDENTIFICATION OF CONJUGATED DRUG METABOLITES [J].

JACOBS, PL ;

DELBRESSINE, LPC ;

KASPERSEN, FM ;

SCHMEITS, GJH .

BIOMEDICAL AND ENVIRONMENTAL MASS SPECTROMETRY, 1987, 14 (11) :689-697

[8] ANALYSIS OF STEROID AND VITAMIN-D GLUCURONIDES AND SULFATES BY FAST ATOM BOMBARDMENT MASS-SPECTROMETRY [J].

JARDINE, I ;

SCANLAN, GF ;

MATTOX, VR ;

KUMAR, R .

BIOMEDICAL MASS SPECTROMETRY, 1984, 11 (01) :4-9

[9] PYRIDINE AS A REAGENT GAS FOR CHARACTERIZATION OF GLUCURONIDES BY CHEMICAL IONIZATION MASS-SPECTROMETRY [J].

JOHNSON, LP ;

RAO, SCS ;

FENSELAU, C .

ANALYTICAL CHEMISTRY, 1978, 50 (14) :2022-2024

[10] CHARACTERIZATION OF GLUCURONIDES WITH A THERMOSPRAY LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY INTERFACE [J].

LIBERATO, DJ ;

FENSELAU, CC ;

VESTAL, ML ;

YERGEY, AL .

ANALYTICAL CHEMISTRY, 1983, 55 (11) :1741-1744