ACTION SPECTRUM FOR CELL KILLING AND PYRIMIDINE DIMER FORMATION IN CHINESE-HAMSTER V-79 CELLS

被引:116
作者
ROTHMAN, RH
SETLOW, RB
机构
[1] Biology Department, Brookhaven National Laboratory, Long Island, New York, 11973, Upton
关键词
D O I
10.1111/j.1751-1097.1979.tb09260.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have determined action spectra for pyrimidine dimer formation and loss of colony‐forming ability in Chinese Hamster V‐79 cells and have found a very strong correlation between the two. These data are consistent with the notion that damage to DNA is the principle cause of cell death and that the most important type of damage is the pyrimidine dimer. While the shape of the V‐79 spectra mimics that of action spectra for bacteria. phage, and purified DNA, V‐79 cells are about twice as sensitive to radiation at long wavelengths, relative to the sensitivity at 265 nm. However, if the action spectra are normalized to 297 nm. a wavelength included in the solar spectrum, the two sets of action spectra would coincide at wavelengths relevant to human skin‐cancer. Thus an action spectrum based on microorganisms should be adequate for extrapolation to humans in terms of risk due to ozone depleteion. Copyright © 1979, Wiley Blackwell. All rights reserved
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页码:57 / 61
页数:5
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