DYNAMIC LIGHT-SCATTERING FROM COLLAGEN SOLUTIONS .2. PHOTON-CORRELATION STUDY OF THE DEPOLARIZED LIGHT

被引：46

作者：

THOMAS, JC ^{[1
]}

FLETCHER, GC ^{[1
]}

机构：

[1] MACQUARIE UNIV, SCH MATH & PHYS, N RYDE 2113, NEW S WALES, AUSTRALIA

来源：

BIOPOLYMERS | 1979年 / 18卷 / 06期

关键词：

D O I：

10.1002/bip.1979.360180603

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The depolarized forward‐scattered light from solutions of rat tail collagen has been studied by photon correlation spectroscopy. The measured autocorrelation function is seen to decay on two widely different time scales. The decay time for the fast component is consistent with the rotational diffusion of rodlike collagen monomers. The slowly decaying autocorrelation component is attributed to large nonspecific aggregates of collagen. A substantial fraction of the collagen is in this aggregated form. Extrapolation of the faster decay times to zero concentration yields a value of θ 20,w0 = 1082 ± 30 sec−1 for the rotational diffusion coefficient of the collagen monomer. Copyright © 1979 John Wiley & Sons, Inc.

引用

页码：1333 / 1352

页数：20

共 38 条

[1] MOLECULAR PARAMETERS OF MONOMERIC AND ACID-SOLUBLE COLLAGENS - LOW SHEAR GRADIENT VISCOSITY AND ELECTRIC BIREFRINGENCE
ANANTHANARAYANAN, S
VEIS, A
[J]. BIOPOLYMERS, 1972, 11 (07) : 1365 - +
[2] Berne B. J., 1976, DYNAMIC LIGHT SCATTE
[3] ELECTRICAL BIREFRINGENCE STUDY OF MONODISPERSE COLLAGEN SOLUTIONS
BERNENGO, JC
ROUX, B
HERBAGE, D
[J]. BIOPOLYMERS, 1974, 13 (03) : 641 - 647
[4] BIANCHI E, 1967, J BIOL CHEM, V242, P1361
[5] THE NATIVE AND DENATURED STATES OF SOLUBLE COLLAGEN
BOEDTKER, H
DOTY, P
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1956, 78 (17) : 4267 - 4280
[6] ROTATIONAL DIFFUSION CONSTANT OF A CYLINDRICAL PARTICLE
BROERSMA, S
[J]. JOURNAL OF CHEMICAL PHYSICS, 1960, 32 (06) : 1626 - 1631
[7] CHIEN JCW, 1975, J MACROMOL SCI R M C, VC 12, P1
[8] MECHANISM OF NUCLEATION FOR INVITRO COLLAGEN FIBRIL FORMATION
COMPER, WD
VEIS, A
[J]. BIOPOLYMERS, 1977, 16 (10) : 2113 - 2131
[9] CHARACTERIZATION OF NUCLEI IN INVITRO COLLAGEN FIBRIL FORMATION
COMPER, WD
VEIS, A
[J]. BIOPOLYMERS, 1977, 16 (10) : 2133 - 2142
[10] TRANSLATIONAL AND ROTATIONAL DIFFUSION CONSTANTS OF TOBACCO MOSAIC VIRUS FROM RAYLEIGH LINEWIDTHS
CUMMINS, HZ
CARLSON, FD
HERBERT, TJ
WOODS, G
[J]. BIOPHYSICAL JOURNAL, 1969, 9 (04) : 518 - &

← 1 2 3 4 →