ABSOLUTE QUANTIFICATION OF P-31 MUSCLE METABOLITES USING NMRS WITH AN INTERNAL STANDARD AND A HIGH-Q, DOUBLE-TUNED COIL

被引：3

作者：

DEBISSCHOP, E

ANNAERT, G

LUYPAERT, R

COREMANS, J

OSTEAUX, M

机构：

[1] Biomedical MR Unit, PRIMIS, Brussels

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1094卷 / 02期

关键词：

ABSOLUTE QUANTIFICATION; ATP; PHOSPHOCREATINE; INORGANIC PHOSPHATE; P-31-NMR; SPECTROSCOPY; (RAT THIGH MUSCLE);

D O I：

10.1016/0167-4889(91)90002-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

P-31-nuclear magnetic resonance spectroscopy (NMRS) is used to determine absolute concentrations of P-31 metabolites in rat muscle. A technique exploiting tissue water H-1 as internal concentration reference and a highly sensitive, double-tuned coil is described and evaluated experimentally. On KH2PO4 solutions of known concentration and varying coil loading it is shown to allow neutralization of systematic errors due to conductive losses, which normally range up to 20% or more. In vivo application in the determination of the absolute concentrations of ATP, PCr and P(i) in eight rat thighs yields results that are in good agreement with literature values.

引用

页码：147 / 152

页数：6

共 7 条

[1] ABSOLUTE QUANTITATION OF PHOSPHORUS METABOLITES IN THE CEREBRAL-CORTEX OF THE NEWBORN HUMAN INFANT AND IN THE FOREARM MUSCLES OF YOUNG-ADULTS USING A DOUBLE-TUNED SURFACE COIL [J].