A NEW PROCEDURE FOR PEPTIDE ALIGNMENT IN PROTEIN-SEQUENCE DETERMINATION USING FAST-ATOM-BOMBARDMENT MASS-SPECTRAL DATA

被引：3

作者：

PETRILLI, P

SEPE, C

PUCCI, P

机构：

[1] UNIV BASILICATA,IST CHIM,VIA PASINI 5,I-80131 NAPLES,ITALY

[2] NAPLES UNIV,CNR,SERV SPETTROMETRIA MASSA,I-80131 NAPLES,ITALY

[3] NAPLES UNIV,IST IND AGR,I-80131 NAPLES,ITALY

[4] NAPLES UNIV,DIPARTIMENTO CHIM ORGAN & BIOL,I-80131 NAPLES,ITALY

来源：

BIOLOGICAL MASS SPECTROMETRY | 1991年 / 20卷 / 03期

关键词：

D O I：

10.1002/bms.1200200304

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A computer program allowing the correct alignment of peptides generated by a first cleaving agent during protein sequence determination studies has been developed. The program elaborates data obtained from fast atom bombardment mass spectrometric analysis of different digests of the protein. The recorded mass values are used to identify peptides in these digests that overlap peptides from the first cleavage, thus making it possible to establish unambiguously the correct order of these peptides in the protein chain. This procedure has been tested on a model protein by reconstructing the complete sequence of human beta-globin chain, determining the correct alignment of 14 tryptic peptides.

引用

页码：115 / 120

页数：6

共 10 条

[1] FAST ATOM BOMBARDMENT OF SOLIDS (FAB) - A NEW ION-SOURCE FOR MASS-SPECTROMETRY [J].