SELECTIVE RECONSTITUTION OF THE TONOPLAST H+-ATPASE OF THE CRASSULACEAN-ACID METABOLISM PLANT KALANCHOE-DAIGREMONTIANA

被引：13

作者：

BEHRE, B ^{[1
]}

RATAJCZAK, R ^{[1
]}

LUTTGE, U ^{[1
]}

机构：

[1] TH DARMSTADT,INST BOT,SCHNITTSPAHNSTR 3-5,W-6100 DARMSTADT,GERMANY

来源：

BOTANICA ACTA | 1992年 / 105卷 / 04期

关键词：

CAM; TONOPLAST; H+-ATPASE; SOLUBILIZATION; RECONSTITUTION; KALANCHOE-DAIGREMONTIANA;

D O I：

10.1111/j.1438-8677.1992.tb00296.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A detergent removal technique was used to reconstitute solubilized tonoplast proteins of mesophyll cells of the CAM plant Kalanchoe daigremontiana into phosphatidylcholine liposomes. The proteoliposomes were able to hydrolyse ATP and to pump protons across the vesicle membrane. Both activities were inhibited by nitrate, an inhibitor of V-type ATPases. Freeze-fracture micrographs confirmed the incorporation of membrane proteins into liposomes. Increase of specific ATP-hydrolysis activity compared to solubilized tonoplast proteins and SDS-PAGE analysis of reconstituted proteins in comparison with the polypeptide pattern of the purified tonoplast H+-ATPase from the same plant source indicated a highly selective reconstitution of the tonoplast H+-ATPase.

引用

页码：260 / 265

页数：6

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