CLONING AND CHARACTERIZATION OF THE GENE ENCODING AN ESTERASE FROM SPIRULINA-PLATENSIS

被引：13

作者：

SALVI, S

TRINEI, M

LANFALONI, L

PON, CL

机构：

[1] UNIV CAMERINO,MCA,DEPT BIOL,I-62032 CAMERINO,ITALY

[2] UNIV PERUGIA,INST CELL BIOL,I-06123 PERUGIA,ITALY

来源：

MOLECULAR & GENERAL GENETICS | 1994年 / 243卷 / 01期

关键词：

MICROALGAE; CYANOBACTERIA; ESTERASE; GENE CLONING; DNA SEQUENCE;

D O I：

10.1007/BF00283885

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene encoding a 23 kDA serine esterase from the cyanobacterium Spirulina platensis has been identified, cloned, characterized and expressed in Escherichia coli. The primary structure of the esterase deduced from the DNA sequence displayed 32% sequence identity with the carboxylesterase (esterase II) encoded by estB of Pseudomonas fluorescens; the highest degree of homology is found in a stretch of 11 identical or highly conserved amino acid residues corresponding to the GXSXG consensus motif found in the catalytic site of many serine proteases, lipases and esterases.

引用

页码：124 / 126

页数：3

共 16 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] THE MOLECULAR EVOLUTION OF GENES AND PROTEINS - A TALE OF 2 SERINES [J].