ISOLATION AND CHARACTERIZATION OF A PSEUDOMONAS STRAIN PRODUCING GLUTARYL-7-AMINOCEPHALOSPORANIC ACID ACYLASE

被引：12

作者：

BINDER, RG ^{[1
]}

NUMATA, K ^{[1
]}

LOWE, DA ^{[1
]}

MURAKAMI, T ^{[1
]}

BROWN, JL ^{[1
]}

机构：

[1] BRISTOL MYERS SQUIBB RES INST,MEGURO KU,TOKYO 153,JAPAN

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1993年 / 59卷 / 10期

关键词：

D O I：

10.1128/AEM.59.10.3321-3326.1993

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Several screening methods were developed for the selection of Pseudomonas strains capable of hydrolyzing glutaryl-7-aminocephalosporanic acid to 7-aminocephalosporanic acid. An isolate exhibiting high acylase activity, designated BL072, was identified as a strain of Pseudomonas diminuta. It grew optimally at pH 7 to 8 and at a temperature of 32 to 40-degrees-C, but acylase activity was highest when the strain was grown at 28-degrees-C. Mutants of BL072 were generated by nitrosoguanidine treatment and screened for increased production of glutaryl-7-aminocephalosporanic acid acylase. A superior mutant gave a fourfold increase in acylase titer. The cell-associated acylase had similar activities against various glutaryl-cephems but had undetectable activity against cephalosporin C. This acylase may prove useful for the conversion of cephalosporin C to 7-aminocephalosporanic acid.

引用

页码：3321 / 3326

页数：6

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