APPLICATION OF ENZYME-FIELD EFFECT TRANSISTOR SENSOR ARRAYS AS DETECTORS IN A FLOW-INJECTION ANALYSIS SYSTEM FOR SIMULTANEOUS MONITORING OF MEDIUM COMPONENTS .2. MONITORING OF CULTIVATION PROCESSES

被引：23

作者：

KULLICK, T

BECK, U

SCHUBERT, J

SCHEPER, T

SCHUGERL, K

机构：

[1] UNIV HANNOVER,INST CHEM TECH,D-30167 HANNOVER,GERMANY

[2] UNIV MUNSTER,INST BIOCHEM,D-48149 MUNSTER,GERMANY

来源：

ANALYTICA CHIMICA ACTA | 1995年 / 300卷 / 1-3期

关键词：

FLOW INJECTION; SENSORS; FIELD EFFECT TRANSISTOR (FET); GLUCOSE DEHYDROGENASE; MALTASE; INVERTASE; BETA-GALACTOSIDASE; GALACTOSE DEHYDROGENASE; ALCOHOL DEHYDROGENASE; ALDEHYDE DEHYDROGENASE; UREASE;

D O I：

10.1016/0003-2670(94)00376-W

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Glucose, maltose, sucrose, lactose, ethanol and urea concentrations were monitored simultaneously during the cultivation of Escherichia coli and Saccharomyces cerevisiae by means of enzyme field effect transistors (EnFETs) applying glucose dehydrogenase (GDH), maltase (MAL)/GDH, invertase (INV)/GDH, beta-galactosidase (beta-GAL)/galactosedehydrogenase (GALDH), alcoholdehydrogenase (ADH)/aldehydedehydrogenase (ALDH), and urease. These enzymes were (co)immobilized on the pH sensitive gates of an eight-FET array. The FET array was integrated in a commercial FIA system.

引用

页码：25 / 31

页数：7