IDENTIFICATION OF A GS-PROTEIN COUPLING DOMAIN TO THE BETA-ADERENOCEPTOR USING SITE-SPECIFIC SYNTHETIC PEPTIDES - CARBOXYL TERMINUS OF GS-ALPHA IS INVOLVED IN COUPLING TO BETA-ADRENOCEPTORS

被引：59

作者：

PALM, D ^{[1
]}

MUNCH, G ^{[1
]}

MALEK, D ^{[1
]}

DEES, C ^{[1
]}

HEKMAN, M ^{[1
]}

机构：

[1] UNIV WURZBURG,DEPT PHYSIOL CHEM,KOELLIKERSTR 2,W-8700 WURZBURG,GERMANY

来源：

FEBS LETTERS | 1990年 / 261卷 / 02期

关键词：

Adrenoceptor; β-; G[!sub]s[!/sub]-protein; Anti-peptide antibody; Coupling domain; Synthetic peptide;

D O I：

10.1016/0014-5793(90)80575-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Competition between Gs-protein and the synthetic peptide, GSA 379-394, derived from the carboxyl-terminal region of the αs-subunit, led to complete inhibition of receptor-mediated adenylate cyclase activation in turkey erythrocyte membranes. Related peptides corresponding to the homologous carboxyl-terminal region of αt-,αil- or αo-subunits did not interfere with β-receptor-Gs coupling. The direct coupling between Gs and adenylate cyclase was not influenced by any of these peptides. These results emphasize the important role of the carboxyl-terminus of G-protein α-subunits for the specific recognition of their corresponding receptors and for signal transduction. © 1990.

引用

页码：294 / 298

页数：5

共 19 条

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