EXTRACELLULAR ATP SHOWS SYNERGISTIC ENHANCEMENT OF DNA-SYNTHESIS WHEN COMBINED WITH AGENTS THAT ARE ACTIVE IN WOUND-HEALING OR AS NEUROTRANSMITTERS

被引：90

作者：

WANG, D

HUANG, NN

HEPPEL, LA

机构：

[1] Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 166卷 / 01期

关键词：

D O I：

10.1016/0006-291X(90)91938-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The polypeptides PDGF, TGFα, and EGF have previously been shown by others to stimulate proliferation of fibroblasts and keratinocytes in the process of wound healing. Here we demonstrate that extracellular ATP, ADP or AMPPNP caused synergistic enhancement of DNA synthesis in 3T6 mouse fibroblasts and BALB MK keratinocytes when combined with any of the above polypeptides. TGFβ showed synergistic stimulation with ATP in fibroblasts but it inhibited keratinocytes. ATP acted as a mitogen for NIE-115 neuroblastoma cultures. In 3T6 cells, ATP stimulated thymidine incorporation in combination with carbachol or norepinephrine. The effect of carbachol was sensitive to atropine. We suggest that extracellular ATP and ADP may play a physiological role in wound healing and as a mitogenic neurotransmitter in the nervous system. © 1990.

引用

页码：251 / 258

页数：8

共 21 条

[1] ACETYLCHOLINE ANALOG STIMULATES DNA-SYNTHESIS IN BRAIN-DERIVED CELLS VIA SPECIFIC MUSCARINIC RECEPTOR SUBTYPES [J].