AN INVITRO CLONOGENIC-ASSAY TO ASSESS RADIATION-DAMAGE IN RAT CNS GLIAL PROGENITOR CELLS

被引：18

作者：

VANDERMAAZEN, RWM

VERHAGEN, I

VANDERKOGEL, AJ

机构：

[1] Institute of Radiotherapy, University of Nijmegen, 6500 HB, Nijmegen

来源：

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY | 1990年 / 58卷 / 05期

关键词：

D O I：

10.1080/09553009014552211

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Normal glial progenitor cells can be isolated from the rat central nervous system (CNS) and cultured in vitro on a monolayer of type-1 astrocytes. These monolayers are able to support and stimulate explanted glial progenitor cells to proliferate. Employing these in vitro interactions of specific glial cell types, an in vivo-in vitro clonogenic assay has been developed. This method offers the possibility to study the intrinsic radiosensitivity, repair and regeneration of glial progenitor cells after in vitro or in vivo irradiation. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：835 / 844

页数：10

共 14 条

[1] LOCALIZATION OF GLIAL FIBRILLARY ACIDIC PROTEIN IN ASTROCYTES BY IMMUNOFLUORESCENCE [J].

BIGNAMI, A ;

ENG, LF ;

DAHL, D ;

UYEDA, CT .

BRAIN RESEARCH, 1972, 43 (02) :429-&

[2] GROWTH OF A RAT NEUROBLASTOMA CELL LINE IN SERUM-FREE SUPPLEMENTED MEDIUM [J].

BOTTENSTEIN, JE ;

SATO, GH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (01) :514-517

[3]

EISENBARTH GS, 1979, P NATL ACAD SCI USA, V76, P4913, DOI 10.1073/pnas.76.10.4913

[4] THE OLIGODENDROCYTE-TYPE-2 ASTROCYTE CELL LINEAGE IS SPECIALIZED FOR MYELINATION [J].

FFRENCHCONSTANT, C ;

RAFF, MC .

NATURE, 1986, 323 (6086) :335-338

[5]

Hopewell JW, 1980, RAD BIOL CANCER RES, P449

[6] PREPARATION OF SEPARATE ASTROGLIAL AND OLIGODENDROGLIAL CELL-CULTURES FROM RAT CEREBRAL TISSUE [J].

MCCARTHY, KD ;

DEVELLIS, J .

JOURNAL OF CELL BIOLOGY, 1980, 85 (03) :890-902

[7] PURIFIED ASTROCYTES PROMOTE THE INVITRO DIVISION OF A BIPOTENTIAL GLIAL PROGENITOR-CELL [J].

NOBLE, M ;

MURRAY, K .

EMBO JOURNAL, 1984, 3 (10) :2243-2247

[8] PLATELET-DERIVED GROWTH-FACTOR FROM ASTROCYTES DRIVES THE CLOCK THAT TIMES OLIGODENDROCYTE DEVELOPMENT IN CULTURE [J].

RAFF, MC ;

LILLIEN, LE ;

RICHARDSON, WD ;

BURNE, JF ;

NOBLE, MD .

NATURE, 1988, 333 (6173) :562-565

[9] A GLIAL PROGENITOR-CELL THAT DEVELOPS INVITRO INTO AN ASTROCYTE OR AN OLIGODENDROCYTE DEPENDING ON CULTURE-MEDIUM [J].

RAFF, MC ;

MILLER, RH ;

NOBLE, M .

NATURE, 1983, 303 (5916) :390-396

[10] GLIAL-CELL DIVERSIFICATION IN THE RAT OPTIC-NERVE [J].

RAFF, MC .

SCIENCE, 1989, 243 (4897) :1450-1455

← 1 2 →