PRODUCTION OF ASPARTAME PRECURSOR BY SEMICONTINUOUS PULSED EXTRACTION COLUMN BIOREACTOR RETAINING FREE ENZYME IN AN AQUEOUS PHASE

被引：6

作者：

HIRATA, A ^{[1
]}

HIRATA, M ^{[1
]}

FURUZAWA, H ^{[1
]}

HONDA, N ^{[1
]}

机构：

[1] CREAT ENGN RES INST,TOKYO 105,JAPAN

来源：

KAGAKU KOGAKU RONBUNSHU | 1991年 / 17卷 / 03期

关键词：

BIOCHEMICAL ENGINEERING; ASPARTAME PRECURSOR; BIOREACTOR; ENZYMATIC SYNTHESIS; PULSED EXTRACTION COLUMN; BUTYL ACETATE; BIPHASIC SYSTEM; FREE ENZYME; THERMOLYSIN;

D O I：

10.1252/kakoronbunshu.17.586

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

A semi-continuous pulsed extraction column bioreactor retaining free enzyme in an aqueous phase was devised for the enzymatic synthesis of the precursor of the artificial sweetener aspartame, using butyl acetate as an organic solvent for substrates feeding and product extraction. Only organic solvent was fed and flowed out, and free enzyme was retained in the reactor without immobilization. This reactor made it possible to use free enzyme continuously and to carry out stable production and extraction by pulsation.

引用

页码：586 / 588

页数：3

共 5 条

[1] SOLVENT EFFECT OF ACETIC ESTERS ON SYNTHESIS OF ASPARTAME PRECURSOR BY FREE ENZYME, ACCOMPANIED BY EXTRACTION
HIRATA, A
HIRATA, M
[J]. KAGAKU KOGAKU RONBUNSHU, 1991, 17 (03) : 511 - 517
[2] HIRATA A, 1987, FINE CHEM, V16, P14
[3] CONTINUOUS SYNTHESIS OF N-(BENZYLOXYCARBONYL)-L-ASPARTYL-L-PHENYLALANINE METHYL-ESTER WITH IMMOBILIZED THERMOLYSIN IN AN ORGANIC-SOLVENT
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KAMIKUBO, T
MATSUNO, R
[J]. BIO-TECHNOLOGY, 1985, 3 (05): : 459 - 464
[4] LONG-TERM CONTINUOUS SYNTHESIS OF ASPARTAME PRECURSOR IN A COLUMN REACTOR WITH AN IMMOBILIZED THERMOLYSIN
NAKANISHI, K
TAKEUCHI, A
MATSUNO, R
[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1990, 32 (06) : 633 - 636
[5] OYAMA K, 1987, METHOD ENZYMOL, V136, P503

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