DEVELOPMENT OF DNA PROBES FOR EARLY DIAGNOSIS AND EPIDEMIOLOGIC-STUDY OF CRYPTOCOCCOSIS IN AIDS PATIENTS

被引:34
作者
POLACHECK, I [1 ]
LEBENS, G [1 ]
HICKS, JB [1 ]
机构
[1] Scripps Res Inst, RES INST, DEPT MOLEC BIOL, LA JOLLA, CA 92037 USA
关键词
D O I
10.1128/JCM.30.4.925-930.1992
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We report the isolation of middle-repetitive DNA sequences from Cryptococcus neoformans that are species and variety specific. These probes were used for assessing strain relatedness among cryptococcal isolates from patients with and without AIDS who were from Zaire and the United States. Five distinct hybridization patterns were observed for the 60 isolates examined, regardless of the restriction enzyme used for digestion. The most common pattern among the isolates from the patients without AIDS was also the most common among the isolates from the patients with AIDS who were from the United States and was the only pattern observed for all isolates tested from patients with AIDS who were from Zaire. On the basis of the high specificity and sensitivity of the signals observed by hybridization, we suggest that these sequences provide a means for both biotyping and early diagnosis of C. neoformans.
引用
收藏
页码:925 / 930
页数:6
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