APPLICATION OF AN INTEGRATED RATE-EQUATION TO THE INACTIVATION OF CATALASE

被引：15

作者：

FEUERS, RJ

PATTILLO, FM

OSBORN, CK

ADAMS, KL

DELUCA, D

SMITH, WG

机构：

[1] UNIV ARKANSAS MED SCI HOSP, DEPT BIOCHEM & MOLEC BIOL, SLOT S16, 4301 W MARKHAM, LITTLE ROCK, AR 72205 USA

[2] NATL CTR TOXICOL RES, JEFFERSON, AR 72079 USA

来源：

FREE RADICAL BIOLOGY AND MEDICINE | 1993年 / 15卷 / 02期

关键词：

CATALASE; INTEGRATED RATE EQUATION; KINETICS; ENZYME INACTIVATION; FREE RADICALS;

D O I：

10.1016/0891-5849(93)90063-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A feature of catalase that has received scant attention in recent years and that may have physiological significance is the peroxide-dependent inactivation of the enzyme. In this article we show how to obtain the second-order rate constant for the inactivation reaction by fitting the reaction progress curve to an integrated rate equation. This method will simplify quantitation of the inactivation and reactivation of catalase. These measurements in tissues with altered metabolic states may reveal new information about the role of catalase in nutrition, aging, and pathology.

引用

页码：223 / 226

页数：4

共 15 条

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