FRACTIONATION OF PLANT EXTRACTS BY THIN-LAYER ELECTROPHORETIC AND CHROMATOGRAPHIC PROCEDURES

被引：22

作者：

COOK, AR

BIELESKI, RL

机构：

[1] Fruit Research Division, D.S.I.R., Auckland

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 28卷 / 1-3期

关键词：

D O I：

10.1016/0003-2697(69)90197-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Methods are described for separating plant tissue extracts into amino acid, organic acid, and neutral fractions by means of thin-layer electrophoresis. The electrophoresis also provides the first-dimensional oeparation for both amino acid and organic acid fractions. Separation of amino acids and organic acids in the second dimension, and of sugars in both dimensions, is achieved by thin-layer chromatography. Suitable procedures for including the study of phospholipids and phosphate esters are suggested. Extracts from 20 mg tissue can be studied easily in this way. © 1969.

引用

页码：428 / &

共 14 条

[1] DETECTION AND DIFFERENTIATION OF SUGARS AND POLYOLS ON SINGLE PAPER CHROMATOGRAMS [J].