Utility of [16 alpha-I-125] iodoestradiol for autoradiography for the study of cellular and regional distribution of receptors

被引：11

作者：

Stumpf, Walter E. ^{[1
,2
]}

Morin, Judy K. ^{[1
,2
]}

Ennis, Bruce W. ^{[1
,2
]}

Zielinski, Jan E. ^{[3
,4
]}

Hochberg, Richard B. ^{[3
,4
]}

机构：

[1] Univ N Carolina, Dept Anat, Chapel Hill, NC 27514 USA

[2] Univ N Carolina, Dept Pharmacol, Chapel Hill, NC 27514 USA

[3] Yale Univ, Sch Med, Dept Obstet & Gynecol, New Haven, CT 06510 USA

[4] Yale Univ, Sch Med, Dept Mol Biophys & Biochem, New Haven, CT 06510 USA

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1987年 / 35卷 / 01期

关键词：

autoradiography; I-125-estradiol; brain; pituitary; uterus;

D O I：

10.1177/35.1.3794310

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We demonstrate the utility of [16 alpha-I-125]iodoestradiol for thaw-mount autoradiography with 2 mu m and 4 mu m thick sections of rat and mouse uterus, pituitary, and brain after in vivo administration. Under the conditions of the experiments, short-term autoradiography with exposure times between 3 and 14 days provides optimal cellular resolution, whereas long-term autoradiography with 1-2 months of exposure may be used to obtain topographic-regional surveys of distribution.

引用

页码：87 / 92

页数：6

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