ISOLATION OF HOMOGENEOUS PHOSPHOLIPASE-A2 FROM HUMAN-PLATELETS

被引：65

作者：

APITZCASTRO, RJ ^{[1
]}

MAS, MA ^{[1
]}

CRUZ, MR ^{[1
]}

JAIN, MK ^{[1
]}

机构：

[1] UNIV DELAWARE,DEPT CHEM,NEWARK,DE 19711

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 91卷 / 01期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0006-291X(79)90583-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phospholipase A2 from human blood platelets has been purified to homogeneity by treatment of the platelet homogenate with H2SO4, followed by affinity chromatography of the extract. The platelet phospholipase is a soluble, heat-stable protein of MW 44000. The purified enzyme inhibits ADP-induced platelet aggregation. © 1979.

引用

页码：63 / 71

页数：9

共 22 条

[1] PLASMA-MEMBRANE PHOSPHORYLATION BY ENDOGENOUS PHOSPHATE DONORS IN HUMAN-BLOOD PLATELETS SELECTIVITY OF ACTION OF DIBUTYRYL CYCLIC-AMP
APITZCASTRO, R
RAMIREZ, E
MAINGON, R
DEMURCIANO, A
RIBBI, A
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1976, 455 (02) : 371 - 382
[2] BARBER AJ, 1970, J BIOL CHEM, V245, P6357
[3] EARLY AND DISCRETE CHANGES IN PERMEABILITY OF ESCHERICHIA-COLI AND CERTAIN OTHER GRAM-NEGATIVE BACTERIA DURING KILLING BY GRANULOCYTES
BECKERDITE, S
MOONEY, C
WEISS, J
FRANSON, R
ELSBACH, P
[J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1974, 140 (02) : 396 - 409
[4] DISTRIBUTION AND METABOLISM OF ARACHIDONIC-ACID IN RABBIT PLATELETS DURING AGGREGATION AND ITS MODIFICATION BY DRUGS
BLACKWELL, GJ
DUNCOMBE, WG
FLOWER, RJ
PARSONS, MF
VANE, JR
[J]. BRITISH JOURNAL OF PHARMACOLOGY, 1977, 59 (02) : 353 - 366
[5] ACTIVITY OF PURE PHOSPHOLIPASE A2 FROM CROTALUS-ATROX VENOM ON MYELIN AND ON PURE PHOSPHOLIPIDS
COLES, E
MCILWAIN, DL
RAPPORT, MM
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1974, 337 (01) : 68 - 78
[6] FRANSON R, 1978, CIRCULATION S2, V57, P124
[7] IATRIDIS SG, 1976, HAEMOSTASIS, V5, P165
[8] JAIN MK, 1978, J BIOL CHEM, V253, P7005
[9] LOW CONCENTRATIONS OF INDOMETHACIN INHIBIT PHOSPHOLIPASE-A2 OF RABBIT POLYMORPHONUCLEAR LEUKOCYTES
KAPLAN, L
WEISS, J
ELSBACH, P
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (06) : 2955 - 2958
[10] KATES M, 1972, LABORATORY TECHNIQUE, V3, P551

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