SEQUENCES ENCODING THE PROTEIN AND RNA COMPONENTS OF RIBONUCLEASE-P FROM STREPTOMYCES-BIKINIENSIS VAR ZORBONENSIS

被引：13

作者：

MORSE, DP ^{[1
]}

SCHMIDT, FJ ^{[1
]}

机构：

[1] UNIV MISSOURI,DEPT BIOCHEM,COLUMBIA,MO 65212

来源：

GENE | 1992年 / 117卷 / 01期

关键词：

ACTINOMYCETES; RECOMBINANT DNA; RNASE-P; COMPLEMENTATION; ESCHERICHIA-COLI; 5' END MAPPING;

D O I：

10.1016/0378-1119(92)90490-G

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The genes encoding the RNA (rnpB) and protein (rnpA) subunits of ribonuclease P (RNase P) of Streptomyces bikiniensis var. zorbonensis have been cloned by complementing the temperature-sensitive growth phenotype of Escherichia coli strains that carry mutations in these genes. The rnpB sequence of S. bikiniensis includes new covariations that lead to refinement of the previous secondary structure models for RNase P RNAs. The deduced amino acid sequence of S. bikiniensis RNase P is conserved with that of other known RNase P proteins only to a limited extent. Immediately upstream from rnpA is an open reading frame that codes for the highly conserved ribosomal protein, L34. This same gene arrangement occurs in all bacteria studied to date.

引用

页码：61 / 66

页数：6

共 25 条

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