MICRO ENZYMATIC ASSAY COUPLED TO CAPILLARY ELECTROPHORESIS VIA LIQUID-JUNCTION

被引：17

作者：

EMMER, A ^{[1
]}

ROERAADE, J ^{[1
]}

机构：

[1] ROYAL INST TECHNOL,DEPT ANALYT CHEM,S-10044 STOCKHOLM,SWEDEN

来源：

CHROMATOGRAPHIA | 1994年 / 39卷 / 5-6期

关键词：

CAPILLARY ELECTROPHORESIS; MICRO ENZYMATIC ASSAY; POSTCOLUMN REACTOR; LIQUID JUNCTION;

D O I：

10.1007/BF02274513

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A system for capillary electrophoresis combined with enzymatic assay has been evaluated for the two enzymes glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase. Instrumentation included a post-column reactor coupled to the separation capillary by a liquid junction. A technique for generating a substrate solution flow into the reactor by utilizing two high voltage supplies is proposed. This method offers a high degree of freedom in optimizing the separation and enzymatic reaction conditions individually. Possibilities for improving the enzymatic assay sensitivity were also examined.

引用

页码：271 / 278

页数：8

共 28 条

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