SELECTIVE ELUTION AND PURIFICATION OF LIVING TRICHOMONAS-VAGINALIS USING GRAVITATIONAL FIELD-FLOW FRACTIONATION

被引：40

作者：

BERNARD, A

BORIES, C

LOISEAU, PM

CARDOT, PJP

机构：

[1] UNIV PARIS 11,CTR ETUD PHARMACEUT,CHIM ANALYT & ELECTROCHIM ORGAN LAB,F-92296 CHATENAY MALABRY,FRANCE

[2] UNIV PARIS 11,CTR ETUD PHARMACEUT,PARASITOL LAB,F-92296 CHATENAY MALABRY,FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 664卷 / 02期

关键词：

D O I：

10.1016/0378-4347(94)00498-T

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Gravitational held-how fractionation is one of the simplest separation methods for biological materials. Its potential in parasitology is demonstrated for Trichomonas vaginalis, a parasite responsible for one of the most widespread sexually transmitted diseases. It was observed that this unicellular parasite can be purified in a culture medium with a recovery of 85% for the living trophozoites. The parasite retention characteristics were different when motile living and non-motile dead cells were eluted, motile cells being less retained than the non-motile cells.

引用

页码：444 / 448

页数：5

共 16 条

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ANDREUX JP, 1993, EXP HEMATOL, V21, P326

[2]

BERNARD A, IN PRESS TRENDS ANAL

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