MAPPING OF ANTIGENIC SITES ON THE BOVINE EPHEMERAL FEVER VIRUS GLYCOPROTEIN USING MONOCLONAL-ANTIBODIES

被引：50

作者：

CYBINSKI, DH

WALKER, PJ

BYRNE, KA

ZAKRZEWSKI, H

机构：

来源：

JOURNAL OF GENERAL VIROLOGY | 1990年 / 71卷

关键词：

D O I：

10.1099/0022-1317-71-9-2065

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Monoclonal antibodies (MAbs) were produced against the G, M2 and N proteins of bovine ephemeral fever virus (BEFV) and 29 were selected for further study. Thirteen neutralizing MAbs were assigned to one conformation-independent and at least two conformation-dependent antigenic sites on the G protein by a competitive binding ELISA. The panel of MAbs were tested by neutralization and immunofluorescence with three strains of BEFV and three BEFV-related viruses. The results indicated that BEFV strains from different sources were not identical and that the M2 prortein was the least variable of the proteins investigated. Passive protection studies in mice showed that the correlataion between neutralizing titre and resistance to challenge was 0.85 (P < 0.001).

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页码：2065 / 2072

页数：8

相关论文

共 26 条

[1] PARAMETERS AFFECTING ASCITES TUMOR-FORMATION IN MICE AND MONOCLONAL-ANTIBODY PRODUCTION [J].

BRODEUR, BR ;

TSANG, P ;

LAROSE, Y .

JOURNAL OF IMMUNOLOGICAL METHODS, 1984, 71 (02) :265-272

[2] ONE-STEP PURIFICATION OF MOUSE MONOCLONAL-ANTIBODIES FROM ASCITIC FLUID BY DEAE AFFI-GEL BLUE CHROMATOGRAPHY [J].

BRUCK, C ;

PORTETELLE, D ;

GLINEUR, C ;

BOLLEN, A .

JOURNAL OF IMMUNOLOGICAL METHODS, 1982, 53 (03) :313-319

[3] CHARACTERIZATION OF A NEW VIRUS-NEUTRALIZING EPITOPE THAT DENOTES A SEQUENTIAL DETERMINANT ON THE RABIES VIRUS GLYCOPROTEIN [J].

BUNSCHOTEN, H ;

GORE, M ;

CLAASSEN, IJTM ;

UYTDEHAAG, FGCM ;

DIETZSCHOLD, B ;

WUNNER, WH ;

OSTERHAUS, ADME .

JOURNAL OF GENERAL VIROLOGY, 1989, 70 :291-298

[4]

CALISHER CH, 1989, INTERVIROLOGY, V50, P241

[5] EPITOPE MAPPING OF JAPANESE ENCEPHALITIS-VIRUS ENVELOPE PROTEIN USING MONOCLONAL-ANTIBODIES AGAINST AN INDIAN STRAIN [J].

CECILIA, D ;

GADKARI, DA ;

KEDARNATH, N ;

GHOSH, SN .

JOURNAL OF GENERAL VIROLOGY, 1988, 69 :2741-2747

[6] ANTIBODIES TO AKABANE VIRUS IN AUSTRALIA [J].

CYBINSKI, DH ;

STGEORGE, TD ;

PAULL, NI .

AUSTRALIAN VETERINARY JOURNAL, 1978, 54 (01) :1-3

[7] THE ISOLATION AND PRELIMINARY CHARACTERIZATION OF A RHABDOVIRUS IN AUSTRALIA RELATED TO BOVINE EPHEMERAL FEVER VIRUS [J].

CYBINSKI, DH ;

ZAKRZEWSKI, H .

VETERINARY MICROBIOLOGY, 1983, 8 (03) :221-235

[8] HOMOLOGOUS AND HETEROLOGOUS ANTIBODY REACTIONS IN SERA FROM CATTLE NATURALLY INFECTED WITH BOVINE EPHEMERAL FEVER GROUP VIRUSES [J].

CYBINSKI, DH .

VETERINARY MICROBIOLOGY, 1987, 13 (01) :1-9

[9] PHYSICOCHEMICAL CHARACTERIZATION OF BOVINE EPHEMERAL FEVER VIRUS AS A MEMBER OF THE FAMILY RHABDOVIRIDAE [J].

DELLAPORTA, AJ ;

BROWN, F .

JOURNAL OF GENERAL VIROLOGY, 1979, 44 (JUL) :99-112

[10] EXPERIMENTAL INACTIVATED VIRUS-VACCINE AGAINST BOVINE EPHEMERAL FEVER .2. DO NEUTRALIZING ANTIBODIES PROTECT AGAINST INFECTION [J].

DELLAPORTA, AJ ;

SNOWDON, WA .

VETERINARY MICROBIOLOGY, 1979, 4 (03) :197-208