USE OF HIGH-PRESSURE LIQUID-CHROMATOGRAPHY (HPLC) FOR THE SEPARATION OF RADIOLABELED ARACHIDONIC-ACID AND ITS METABOLITES PRODUCED BY THROMBIN-TREATED HUMAN-PLATELETS .2. ESTABLISHMENT OF OPTIMAL ASSAY CONDITIONS

被引：24

作者：

DEYKIN, D

RUSSELL, FA

VAILLANCOURT, R

机构：

[1] BOSTON UNIV,DEPT MED,BOSTON,MA 02215

[2] TUFTS UNIV,SCH MED,DEPT MED,BOSTON,MA 02111

来源：

PROSTAGLANDINS | 1979年 / 18卷 / 01期

关键词：

D O I：

10.1016/S0090-6980(79)80019-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have utilized HPLC to develop optimal conditions for assaying the transformation of arachidonic acid in thrombin-treated human platelets. In the presence of increasing amounts of albumin, the total amount of radioactivity released from thrombin-treated platelets pre-labeled with 3H-arachidonic acid is first enhanced and then inhibited. Maximal release, reflecting primarily enhanced amounts of free labeled arachidonic acid, occurs at a final albumin concentration of 0.5 mg/ml. Calcium promoted the release of all radiolabeled metabolites, but it specifically enhanced HETE formation and release. Magnesium was without effect. Cyclo-oxygenase derived products constituted the bulk of released label at short time intervals, but after ten minutes exposure to thrombin in the presence of albumin (0.5 mg/ml) and 3 mM calcium, radioactivity in the released products was equally distributed among cyclo-oxygenase derived products (TXB2 + PGD2 + HHT), HETE and free arachidonic acid. © 1979 Geron-X, Inc.

引用

页码：19 / 27

页数：9

共 14 条

[1]

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[2]

COOPER B, 1978, BLOOD, V52, P618

[3]

DEYKIN D, 1978, CLIN RES, V26, pA503

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