CYTOSKELETON ARCHITECTURE OF C6 RAT GLIOMA CELL SUBCLONES DIFFERING IN INTERMEDIATE FILAMENT PROTEIN EXPRESSION

被引:21
作者
BOHN, W [1 ]
ROSER, K [1 ]
HOHENBERG, H [1 ]
MANNWEILER, K [1 ]
TRAUB, P [1 ]
机构
[1] MAX PLANCK INST CELL BIOL,LADENBURG,GERMANY
关键词
D O I
10.1006/jsbi.1993.1035
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Whole-mount electron microscopy was used in conjunction with immunogold labeling to characterize the cytoskeleton architecture of C6 rat glioma cell subclones. These subclones differ in intermediate filament (IF) protein composition and either contain vimentin (subclone C6D8) or do not express any of the known cytoplasmic IF proteins (subclone C6D10) (Roser et al., 1991). In C6D8 cells short thin (3 nm) connecting filaments frequently linked vimentin to actin filaments and, in addition, connected vimentin filaments to each other. Occasionally, direct contacts were noticed between actin and vimentin filaments. Thin connecting filaments were present at a significantly higher number in IF-deficient C6D10 cells, forming a dense cytoplasmic network in conjunction with actin filament bundles as the dominating structure. The data indicate that thin connecting filaments are present in C6 cells independent of the expression of cytoplasmic IF proteins. They suggest that structural linkages between vimentin and actin filaments mediated by thin connecting filaments could play a major role in the determining the cytoskeleton architecture of these cells. © 1993 by Academic Press, Inc.
引用
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页码:48 / 58
页数:11
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