IMPROVED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF POLYCYCLIC AROMATIC HYDROCARBON METABOLITES IN HUMAN URINE

被引：28

作者：

WHITON, RS ^{[1
]}

WITHERSPOON, CL ^{[1
]}

BUCKLEY, TJ ^{[1
]}

机构：

[1] US EPA,HUMAN EXPOSURE RES BRANCH,RES TRIANGLE PK,NC 27711

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 665卷 / 02期

关键词：

D O I：

10.1016/0378-4347(94)00537-F

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reversed-phase HPLC method with fluorescence detection was evaluated for utility in determination of urinary metabolites of polycyclic aromatic hydrocarbons as biomarkers of environmental exposure. The method, which was developed for use in studies of high-level occupational exposure, was found to be unreliable for relatively low-level environmental exposures. The method was modified to include quantitation by standard addition in order to compensate for matrix effects at levels as low as 0.1 ng/ml. The standard addition modification increased both qualitative and quantitative performance, with recovery of 1-hydroxypyrene spikes improved from 164% to 114% at 0.36 ng/ml. The modified method was successfully applied in an environmental exposure study.

引用

页码：390 / 394

页数：5

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