CATALASE EXPRESSION, KATG, AND MIC OF ISONIAZID FOR MYCOBACTERIUM-TUBERCULOSIS ISOLATES FROM SAO-PAULO, BRAZIL

被引：14

作者：

FERRAZOLI, L

PALACI, M

TELLES, MAD

UEKI, SY

KRITSKI, A

MARQUES, LRM

FERREIRA, OC

RILEY, LW

机构：

[1] INST ADOLFO LUTZ REGISTRO,SETOR MICROBACTERIAS,SAO PAULO,BRAZIL

[2] INST INFECTOL EMILIO RIBAS,SAO PAULO,BRAZIL

[3] HOSP UNIV CLEMENTINO FRAGA,RIO JANEIRO,BRAZIL

[4] CORNELL UNIV,COLL MED,DIV INT MED,NEW YORK,NY

来源：

JOURNAL OF INFECTIOUS DISEASES | 1995年 / 171卷 / 01期

关键词：

D O I：

10.1093/infdis/171.1.237

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The MIC of isoniazid, peroxidase-catalase expression, and the presence of the katG gene for 102 Mycobacterium tuberculosis isolates from patients in Sao Paulo were compared. Fifty-three isoniazid-resistant and 49 isoniazid-sensitive isolates were analyzed by polymerase chain reaction (PCR) for the presence of katG sequences. All isoniazid-sensitive and 43 (81%) isoniazid-resistant isolates expressed catalase (P = .001). None of isoniazid-sensitive and 4 (7%) of 53 isoniazid-resistant isolates lacked katG sequences. Among 6 isolates with MICs >50 mu g/mL, 5 (83%) did not express catalase and 2 lacked katG sequences; only 1 had complete gene deletion shown by Southern blot analysis. These findings indicate a correlation between loss of catalase and isoniazid resistance among highly resistant isolates, but these isolates were a small proportion of resistant clinical M. tuberculosis isolates from Sao Paulo.

引用

页码：237 / 240

页数：4

共 16 条

[1] MUTATIONS IN THE CATALASE-PEROXIDASE GENE FROM ISONIAZID-RESISTANT MYCOBACTERIUM-TUBERCULOSIS ISOLATES
ALTAMIRANO, M
MAROSTENMAKI, J
WONG, A
FITZGERALD, M
BLACK, WA
SMITH, JA
[J]. JOURNAL OF INFECTIOUS DISEASES, 1994, 169 (05) : 1162 - 1165
[2] INHA, A GENE ENCODING A TARGET FOR ISONIAZID AND ETHIONAMIDE IN MYCOBACTERIUM-TUBERCULOSIS
BANERJEE, A
DUBNAU, E
QUEMARD, A
BALASUBRAMANIAN, V
UM, KS
WILSON, T
COLLINS, D
DELISLE, G
JACOBS, WR
[J]. SCIENCE, 1994, 263 (5144) : 227 - 230
[3] COHN ML, 1954, AM REV TUBERC PULM, V70, P641
[4] DAVID HL, 1986, METHODS LABORATOIRE
[5] PURIFICATION AND PROPERTIES OF PEROXIDASE IN MYCOBACTERIUM-TUBERCULOSIS H37RV AND ITS POSSIBLE ROLE IN MECHANISM OF ACTION OF ISONICOTINIC-ACID HYDRAZIDE
DEVI, BG
SHAILA, MS
RAMAKRISHNAN, T
GOPINATHAN, KP
[J]. BIOCHEMICAL JOURNAL, 1975, 149 (01) : 187 - 197
[6] ISOLATION AND CHARACTERIZATION OF CATALASE PRODUCED BY MYCOBACTERIUM-TUBERCULOSIS
DIAZ, GA
WAYNE, LG
[J]. AMERICAN REVIEW OF RESPIRATORY DISEASE, 1974, 110 (03): : 312 - 319
[7] GOPINATHAN KP, 1981, CURR SCI INDIA, V50, P216
[8] HEDGECOCK LW, 1957, AM REV TUBERC PULM, V75, P670
[9] MODE OF ACTION OF ANTITUBERCULOUS DRUGS AND MECHANISMS OF DRUG-RESISTANCE IN MYCOBACTERIUM-TUBERCULOSIS
RASTOGI, N
DAVID, HL
[J]. RESEARCH IN MICROBIOLOGY, 1993, 144 (02) : 133 - 143
[10] Silva Eny Aparecida Matheus Da, 1992, Revista do Instituto Adolfo Lutz, V52, P37

← 1 2 →