DEVELOPMENT OF A MONOCLONAL ANTIBODY-BASED ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR THE DETECTION OF LIVE PROPAGULES OF TRICHODERMA-HARZIANUM IN A PEAT-BRAN MEDIUM

被引：17

作者：

THORNTON, CR ^{[1
]}

DEWEY, FM ^{[1
]}

GILLIGAN, CA ^{[1
]}

机构：

[1] UNIV CAMBRIDGE,DEPT PLANT SCI,CAMBRIDGE CB2 3EA,ENGLAND

来源：

SOIL BIOLOGY & BIOCHEMISTRY | 1994年 / 26卷 / 07期

关键词：

D O I：

10.1016/0038-0717(94)90307-7

中图分类号：

S15 [土壤学];

学科分类号：

0903 ; 090301 ;

摘要：

Mouse monoclonal antibodies (MAbs) and mouse and rabbit (polyclonal) antisera were raised against a Trichoderma harzianum isolate T95. A cell line, Th.HD3(3), raised from splenocytes from a mouse immunized with lyophilized mycelium and Quil A adjuvant, produced MAbs of the immunoglobulin sub-class IgG2a that were specific recognizing, by ELISA, antigens from a number of Trichoderma species and species from the related genus Hypocrea but not from a range of other soil-borne fungi. These MAbs were used to develop an enzyme-linked immunosorbent assay (ELISA) for the detection of live propagules of Trichoderma harzianum in a peat bran medium. Heat, protease and periodate treatment of the T95 antigen and binding on Western blots indicate that the Th.HD3(3) MAbs recognized a protein epitope of ca M(r) 18,500 which forms part of a larger glycoprotein molecule.

引用

页码：909 / 920

页数：12

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