ELECTRON MICROSCOPY OF POKEWEED LEAF CELLS INFECTED WITH POKEWEED MOSAIC VIRUS

Cytoplasmic inclusions associated with pokeweed mosaic virus (PMV) infection are similar to those which have been reported for other long, flexuous rod-shaped viruses. Cylindrical inclusions and their derivatives such as circular inclusions, tubes, laminated aggregates, bundles, and pinwheels are all associated with PMV infection. A close association between endoplasmic reticulum and both laminated aggregates and bundles was noted. Virus particles were not associated with virus-induced cytoplasmic inclusions but were evident in masses in the cytoplasm near the central vacuole. Near the accumulation of virus particles were many vesicles in various sizes. Golgi apparatus were closely associated with the vesicles. Many secondary vacuoles protruded into the central vacuole. The thin layers of cytoplasm surrounding secondary vacuoles were filled with virus particles. Stellate, crystallike inclusions were noted in chloroplasts of infected cells and apparently were not viral in nature. Cytoplasmic invaginations containing mitochondria or endoplasmic reticulum in chloroplasts of infected cells were noted. © 1969.