INVITRO EFFECT OF LARVAL SCHISTOSOMA-MANSONI EXCRETORY SECRETORY PRODUCTS ON PHAGOCYTOSIS-STIMULATED SUPEROXIDE PRODUCTION IN HEMOCYTES FROM BIOMPHALARIA-GLABRATA

被引:75
作者
CONNORS, VA
YOSHINO, TP
机构
关键词
D O I
10.2307/3282811
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
The in vitro production of the reactive oxygen metabolite superoxide (O2-) was confirmed in hemocytes from the schistosome intermediate host Biomphalaria glabrata. Active forms of the enzyme superoxide dismutase (SOD) inhibited reduction of nitroblue tetrazolium (NBT) to formazan in cells that had phagocytozed zymosan particles, whereas an inactivated form of SOD did not. Moreover, based on the prevalence of O2(-)-positive hemocytes and the relative intensity of NBT staining reactions, hemocytes from the Schistosoma mansoni-resistant 10-R2 strain of B. glabrata possessed an overall greater capacity for generating superoxide than did those from S. mansoni-susceptible M-line snails. Schistosoma mansoni excretory-secretory (E-S) products, released during in vitro transformation of miracidia to sporocysts, inhibited phagocytosis of zymosan particles and superoxide activity in hemocytes from both snail strains, but 10-R2 hemocytes maintained higher levels of phagocytosis and superoxide production than did M-line hemocytes. The dose-dependent decreases in phagocytosis observed in both snail strains in the presence of E-S products could not account fully for the concomitant decrease in superoxide levels detected, indicating that either a single E-S factor differentially affects phagocytosis and superoxide production, or that different E-S factors are involved in the specific interference of each of these hemocyte functions.
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页码:895 / 902
页数:8
相关论文
共 39 条
[1]   BIOMPHALARIA-GLABRATA AMEBOCYTES - EFFECT OF SCHISTOSOMA-MANSONI INFECTION ON INVITRO PHAGOCYTOSIS [J].
ABDULSALAM, JM ;
MICHELSON, EH .
JOURNAL OF INVERTEBRATE PATHOLOGY, 1980, 35 (03) :241-248
[2]   ACTIVE OXYGEN SPECIES AND THE FUNCTIONS OF PHAGOCYTIC LEUKOCYTES [J].
BADWEY, JA ;
KARNOVSKY, ML .
ANNUAL REVIEW OF BIOCHEMISTRY, 1980, 49 :695-726
[3]  
BAYNE CJ, 1989, AM ZOOL, V29, P399
[4]   SCHISTOSOMA-MANSONI - CYTO-TOXICITY OF HEMOCYTES FROM SUSCEPTIBLE SNAIL HOSTS FOR SPOROCYSTS IN PLASMA FROM RESISTANT BIOMPHALARIA-GLABRATA [J].
BAYNE, CJ ;
BUCKLEY, PM ;
DEWAN, PC .
EXPERIMENTAL PARASITOLOGY, 1980, 50 (03) :409-416
[5]   SUPEROXIDE DISMUTASE - IMPROVED ASSAYS AND AN ASSAY APPLICABLE TO ACRYLAMIDE GELS [J].
BEAUCHAM.C ;
FRIDOVIC.I .
ANALYTICAL BIOCHEMISTRY, 1971, 44 (01) :276-&
[6]   SCHISTOSOMA-MANSONI - LECTIN-DEPENDENT CYTO-TOXICITY OF HEMOCYTES FROM SUSCEPTIBLE HOST SNAILS, BIOMPHALARIA-GLABRATA [J].
BOSWELL, CA ;
BAYNE, CJ .
EXPERIMENTAL PARASITOLOGY, 1985, 60 (01) :133-138
[7]   BIOCHEMISTRY OF OXYGEN-TOXICITY [J].
CADENAS, E .
ANNUAL REVIEW OF BIOCHEMISTRY, 1989, 58 :79-110
[8]   ACID-PHOSPHATASE IN GRANULOCYTIC CAPSULES FORMED IN STRAINS OF BIOMPHALARIA-GLABRATA TOTALLY AND PARTIALLY RESISTANT TO SCHISTOSOMA-MANSONI [J].
CHENG, TC ;
GARRABRANT, TA .
INTERNATIONAL JOURNAL FOR PARASITOLOGY, 1977, 7 (06) :467-472
[9]   OBSERVATIONS ON HEARTS EXPLANTED IN VITRO FROM SNAIL AUSTRALORBIS GLABRATUS [J].
CHERNIN, E .
JOURNAL OF PARASITOLOGY, 1963, 49 (03) :353-&
[10]   SCHISTOSOMA-MANSONI - EFFECT OF INFECTION ON REPRODUCTION AND GONADAL GROWTH IN BIOMPHALARIA-GLABRATA [J].
CREWS, AE ;
YOSHINO, TP .
EXPERIMENTAL PARASITOLOGY, 1989, 68 (03) :326-334