CAPILLARY ELECTROPHORESIS WITH SURFACE COATED CAPILLARIES

被引：61

作者：

KOHR, J ^{[1
]}

ENGELHARDT, H ^{[1
]}

机构：

[1] UNIV SAARLAND,W-6600 SAARBRUCKEN,GERMANY

来源：

JOURNAL OF MICROCOLUMN SEPARATIONS | 1991年 / 3卷 / 06期

关键词：

CAPILLARY ELECTROPHORESIS; POLYMER COATED CAPILLARIES; ELECTROOSMOTIC FLOW;

D O I：

10.1002/mcs.1220030603

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

With uncoated fused silica capillaries, the electroosmotic flow and its contribution to solute migration are in part responsible for problems with the reproducibility of migration time due to hysteresis effects. In transferring coating procedures from silica to capillary surfaces, it has been possible to prepare stable polymeric coatings where hysteresis effects are negligible. Acrylamide coatings reduce the electroosmotic flow. A pH independent flow could be achieved with bonded cation exchange groups. With bonded anion exchangers, reversal of electroosmotic flow is possible.

引用

页码：491 / 495

页数：5

共 13 条

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