CELL-GROWTH ON IMMOBILIZED CELL-GROWTH FACTOR .8. PROTEIN-FREE CELL-CULTURE ON INSULIN-IMMOBILIZED MICROCARRIERS

In order to develop a new protein-free cell culture system, microcarriers immobilized with insulin were synthesized. For the synthesis, glass and polyacrylamide beads were treated for the introduction of amino groups on the surface, and insulin was immobilized on the surface by using several methods. Anchorage-dependent cells, mouse fibroblast cells STO and fibroic sarcoma cells HSDM1C1, and the anchorage-independent cells, mouse hybridoma cells SJK132-20 and RDP 45/20 were cultivated on the microcarriers immobilized with insulin. The insulin-immobilized microcarriers did not have any effect on the proliferation of the anchorage-independent cells but promoted the growth of anchorage-dependent cells remarkably. The activity of immobilized insulin was larger than that of free or adsorbed insulin. The repeated use of the insulin-immobilized microcarrier was possible, and the promotion activity in the repeated use was greater than that in the first use.