Detection of groundnut rosette umbravirus infections with radioactive and non-radioactive probes to its satellite RNA

被引：11

作者：

Blok, VC ^{[1
]}

Ziegler, A ^{[1
]}

Scott, K ^{[1
]}

Dangora, DB ^{[1
]}

Robinson, DJ ^{[1
]}

Murant, AF ^{[1
]}

机构：

[1] SCOTTISH CROP RES INST,DUNDEE DD2 5DA,SCOTLAND

来源：

ANNALS OF APPLIED BIOLOGY | 1995年 / 127卷 / 02期

关键词：

groundnut rosette virus; umbravirus; satellite RNA; detection; dot-blots; non-radioactive probes;

D O I：

10.1111/j.1744-7348.1995.tb06676.x

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A cloned cDNA copy of the satellite RNA of groundnut rosette virus (GRV), labelled with either P-32 or digoxigenin, was used as a probe to detect the satellite RNA in infected leaves. The test was successfully applied to N. benthamiana and to groundnuts, infected with isolates of GRV from East and West Africa and with isolates which cause different types of symptom in groundnuts, including one which is almost symptomless. Although the probe did not react with extracts from plants infected with GRV isolates from which the satellite RNA had been artificially eliminated, all naturally occurring GRV isolates contain the satellite RNA. The test therefore provides a reliable indicator of infection by GRV.

引用

页码：321 / 328

页数：8

共 18 条

[1] SEQUENCES OF 10 VARIANTS OF THE SATELLITE-LIKE RNA-3 OF GROUNDNUT ROSETTE VIRUS [J].