A COMPARATIVE-STUDY ON THE EFFECT OF HYDROCHLORIC, PHOSPHORIC, AND TRIFLUOROACETIC-ACID IN THE REVERSED-PHASE CHROMATOGRAPHY OF ANGIOTENSINS AND RELATED PEPTIDES

被引：6

作者：

CORRADINI, D

CANNARSA, G

机构：

[1] Istituto di Crotnatografla del CNR, Area delle Ricerca di Roma, Rome, P.O. box 10

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1995年 / 18卷 / 18-19期

关键词：

D O I：

10.1080/10826079508014632

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper examines the effect of hydrochloric acid as an alternative mobile phase additive in reversed phase chromatography of closely related peptides, such as the analogs of human angiotensin I and III, in comparison to the retention behavior and selectivity obtained with the two most popular additives TFA and phosphoric acid. The effect of the concentration of the additive and of the apparent pH of the mobile phase was investigated by isocratic elution of the selected peptides on a totally porous butyl-silica column with water-acetonitrile eluents containing either additive at various concentrations, corresponding to different values of the apparent pH of the mobile phase within the range 1.23 to 2.28. Differences in retention behavior and selectivity observed with the three mobile phase additives were discussed in terms of differences in the ion-pair associations between the ionized peptides and the counterions in the mobile phase.

引用

收藏

页码：3919 / 3931

页数：13

相关论文

共 18 条

[1] COMPARISON OF REVERSED-PHASE COLUMNS FOR THE SEPARATION OF TRYPTIC PEPTIDES BY GRADIENT ELUTION - CORRELATION OF EXPERIMENTAL RESULTS AND MODEL PREDICTION [J].

BANES, AJ ;

LINK, GW ;

SNYDER, LR .

JOURNAL OF CHROMATOGRAPHY, 1985, 326 (JUN) :419-431

[2] SURFACE SILANOLS IN SILICA-BONDED HYDROCARBONACEOUS STATIONARY PHASES .2. IRREGULAR RETENTION BEHAVIOR AND EFFECT OF SILANOL MASKING [J].

BIJ, KE ;

HORVATH, C ;

MELANDER, WR ;

NAHUM, A .

JOURNAL OF CHROMATOGRAPHY, 1981, 203 (JAN) :65-84

[3]

Corbridge DEC., 1990, PHOSPHORUS OUTLINE I, P959

[4]

FREISER H H, 1987, Biochromatography, V2, P186

[5] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF AMINO-ACIDS, PEPTIDES AND PROTEINS .48. RETENTION BEHAVIOR OF TRYPTIC PEPTIDES OF HUMAN GROWTH-HORMONE ISOLATED BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - A COMPARATIVE-STUDY USING DIFFERENT CHROMATOGRAPHIC CONDITIONS AND PREDICTED ELUTION BEHAVIOR BASED ON RETENTION COEFFICIENTS [J].

GREGO, B ;

LAMBROU, F ;

HEARN, MTW .

JOURNAL OF CHROMATOGRAPHY, 1983, 266 (AUG) :89-103

[6]

GUO D, 1987, J CHROMATOGR, V386, P205

[7] HIGH-PRESSURE LIQUID-CHROMATOGRAPHY OF PEPTIDES AND PROTEINS .6. RAPID ANALYSIS OF PEPTIDES BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY WITH HYDROPHOBIC ION-PAIRING OF AMINO-GROUPS [J].

HANCOCK, WS ;

BISHOP, CA ;

MEYER, LJ ;

HARDING, DRK ;

HEARN, MTW .

JOURNAL OF CHROMATOGRAPHY, 1978, 161 (NOV) :291-298

[8] HIGH-PRESSURE LIQUID-CHROMATOGRAPHY OF PEPTIDES AND PROTEINS .11. USE OF CATIONIC REAGENTS FOR THE ANALYSIS OF PEPTIDES BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY [J].

HANCOCK, WS ;

BISHOP, CA ;

BATTERSBY, JE ;

HARDING, DRK ;

HEARN, MTW .

JOURNAL OF CHROMATOGRAPHY, 1979, 168 (02) :377-384

[9] HIGH-PRESSURE LIQUID-CHROMATOGRAPHY OF PEPTIDES AND PROTEINS .2. USE OF PHOSPHORIC-ACID IN ANALYSIS OF UN-DERIVATIZED PEPTIDES BY REVERSED-PHASE HIGH-PRESSURE LIQUID-CHROMATOGRAPHY [J].

HANCOCK, WS ;

BISHOP, CA ;

PRESTIDGE, RL ;

HARDING, DRK ;

HEARN, MTW .

JOURNAL OF CHROMATOGRAPHY, 1978, 153 (02) :391-398

[10] REVERSED-PHASE, HIGH-PRESSURE LIQUID-CHROMATOGRAPHY OF PEPTIDES AND PROTEINS WITH ION-PAIRING REAGENTS [J].

HANCOCK, WS ;

BISHOP, CA ;

PRESTIDGE, RL ;

HARDING, DRK ;

HEARN, MTW .

SCIENCE, 1978, 200 (4346) :1168-1170