CELL-DIFFERENTIATION RATES OF FRIEND MURINE ERYTHROLEUKEMIA VARIANTS ISOLATED BY SIB SELECTION

被引：8

作者：

AXELROD, DE

TERRY, R

KERN, FG

机构：

[1] Department of Biological Sciences, Douglass Campus, Rutgers-The State University, New Brunswick, 08903, New Jersey

来源：

SOMATIC CELL GENETICS | 1979年 / 5卷 / 05期

关键词：

D O I：

10.1007/BF01542693

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Variants of Friend erythroleukemia cells were isolated which produced a high frequency (98%) or low frequency (2%) of hemoglobinized cells after induction with dimethylsulfoxide. Repeated subcloning and sib selection allowed enrichment of different cell lines without the use of mutagens or drug selection. The cell lines do not differ in growth rates, plating efficiencies, or chromosome numbers. The differences in inducibility phenotype were stable for more than 260 cell generations. In addition to differences in induction by dimethylsulfoxide and butyric acid, the cell lines also differ in spontaneous rates of cell differentiation. These results suggest that differences in differentiation rates are an inherited property of cells which is amplified in the presence of nonphysiological inducing agents. © 1979 Plenum Publishing Corporation.

引用

页码：539 / 549

页数：11

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