EVALUATION OF HLA-CLASS-II IDENTITY BETWEEN UNRELATED INDIVIDUALS BY SEROLOGICAL TYPING, DNA-RFLP METHOD, AND MIXED LYMPHOCYTE-REACTION

被引:23
作者
ALDACCAK, R [1 ]
LOISEAU, P [1 ]
MIRAMONT, P [1 ]
RABIAN, C [1 ]
RAFFOUX, C [1 ]
COLOMBANI, J [1 ]
机构
[1] HOP ST LOUIS,IMMUNOL & HISTOCOMPATIBIL LAB,1 RUE CLAUDE VELLEFAUX,F-75475 PARIS 10,FRANCE
关键词
D O I
10.1016/0198-8859(90)90114-5
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Seven groups, each consisting of two to nine unrelated HLA-A, -B, and -DR serologically identical individuals, were analyzed by DNA-restriction fragment length polymorphism (RFLP) and mixed lymphocyte reactions (MLR) in order to evaluate HLA-class II identity between unrelated individuals and to asses the importance of HLA-class II incompatibilities detected by DNA-RFLP in the allogeneic reactions. It is clear that DNA-RFLP represents a powerful typing method for HLA-DR, -DQ, and -DP ssince the combinations of the RFLP band patterns define all the serological specificities and most of the cellular specificities togive a highly accurate typing. This report shows that an HLA-DP incompatibility induces proliferation in primary mixed lymphocyte culture (MLC) between unrelated HLA-A, -B, -DR, -DQ, and -DW identical individuals, which may suggest the imortance of this molecule as a transplantation antigen, especially for unrelated bone m̀arrow transplantations. Still, an isolated HLA-DPw4/HLA-DPa disparity did not induce any proliferation in MLC. Moreover, our results show that DQw7 (w3)/DQw8 (w3) disparity associated with HLA-DR4 represents a nonfunctional incompatibility in MLR. The HLA-Dw subtypes of HLA-DR specificities can induce a high proliferative response in MLC. Finally, DNA-RFLP typing represents a reliable method for the selection of histocompatible donor-recipient pairs and could potentially reduce many logistic problems and delays in live-donor transplantation, especially for unrelated bone marrow transplantation. © 1990.
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页码:189 / 201
页数:13
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