COMPETITIVE ELUTION OF LACTATE-DEHYDROGENASE FROM CIBACRON BLUE BEAD CELLULOSE WITH CIBACRON BLUE DEXTRANS

被引：7

作者：

MISLOVICOVA, D ^{[1
]}

GEMEINER, P ^{[1
]}

STRATILOVA, E ^{[1
]}

HORVATHOVA, M ^{[1
]}

机构：

[1] SLOVAK ACAD SCI, CTR PHYSIOL SCI, BIOCHEM LAB, CS-83306 BRATISLAVA, CZECHOSLOVAKIA

来源：

JOURNAL OF CHROMATOGRAPHY | 1990年 / 510卷

关键词：

D O I：

10.1016/S0021-9673(01)93753-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The efficiencies of elution of lactate dehydrogenase (LDH) from Cibacron Blue (CB)-bead cellulose with eluents ensuring competitive (Cibacron Blue-dextran), biomimetic NADH) and displacing (KCl) mechanisms were compared. Competitive elution with CB-dextran T 10 was shown to be the most effective providing a 38 fold purified enzyme in 83% yield. As shown by fast protein liquid chromatography and polyacrylamide gel electrophoresis, this LDH preparation was free from protein contaminants but contained CB-dextran. CB-dextran was then removed by ion-exchange chromatography and the yield of LDH decreased to 62%. When using a longer column, the enzyme was resolved partially in two fractions. The isoelectric point of the main fraction was 7.3. © 1990.

引用

页码：197 / 204

页数：8

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