Kinetics of Phosphorus Absorption by Corynebacterium bovis

The initial rate of phosphorus uptake by phosphorus-limited cells of Corynebacterium bovis grown in batch culture and in a chemostat was measured with [P-32] orthophosphate. It was dependent on the external phosphorus concentration and was inversely related to the amount of intracellular phosphorus. The relationship between the initial rate of uptake, intracellular phosphorus, and phosphorus concentration in the medium can be expressed in terms of Haldane's modification of the Michaelis-Menten equation.