CLONING AND CHARACTERIZATION OF A GENE ENCODING EXTRACELLULAR METALLOPROTEASE FROM STREPTOMYCES-LIVIDANS

被引：20

作者：

LICHENSTEIN, HS

BUSSE, LA

SMITH, GA

NARHI, LO

MCGINLEY, MO

ROHDE, MF

KATZOWITZ, JL

ZUKOWSKI, MM

机构：

[1] Amgen Inc., Thousand Oaks

来源：

GENE | 1992年 / 111卷 / 01期

关键词：

ZINC-BINDING MOTIF; LYSR FAMILY; C-14]ALPHA-CASEIN ASSAY; PHOSPHORAMIDON; DNA-BINDING MOTIF; 1,10-PHENANTHROLINE; SECRETION; SIGNAL SEQUENCE; PLASMID PIJ699;

D O I：

10.1016/0378-1119(92)90613-T

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The prt gene, encoding a protease (Prt) from Streptomyces lividans TK24, was cloned and sequenced. An S. lividans host with plasmid-borne prt secreted 200-mu-g/ml of a 22-kDa Prt into the culture medium. Prt is classified as a metalloprotease since its activity is significantly inhibited by 1,10-phenanthroline or EDTA. The region upstream from prt codes for an incomplete open reading frame (ORF) oriented opposite to prt. This ORF has a strong similarity to a gene family (lysR) whose members regulate the transcription of structural genes required for either biosynthesis or degradation.

引用

页码：125 / 130

页数：6

共 36 条

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