THE MOLECULAR CHAPERONE TF55 - ASSESSMENT OF SYMMETRY

被引：56

作者：

MARCO, S

URENA, D

CARRASCOSA, JL

WALDMANN, T

PETERS, J

HEGERL, R

PFEIFER, G

SACKKONGEHL, H

BAUMEISTER, W

机构：

[1] UNIV AUTONOMA MADRID,CSIC,CTR NACL BIOTECNOL,E-28049 MADRID,SPAIN

[2] MAX PLANCK INST BIOCHEM,D-82152 MARTINSRIED,GERMANY

[3] MAX PLANCK GESELL,FRITZ HABER INST,W-1000 BERLIN 33,GERMANY

[4] UNIV AUTONOMA MADRID,CSIRO,CTR BIOL MOLEC,MADRID 34,SPAIN

来源：

FEBS LETTERS | 1994年 / 341卷 / 2-3期

关键词：

MOLECULAR CHAPERONE; ROTATIONAL SYMMETRY; THERMOPHILIC FACTOR (TF55);

D O I：

10.1016/0014-5793(94)80447-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

TF55-like factor from Sulfolobus solfataricus was purified to homogeneity and analyzed by electron microscopy and image analysis to determine the symmetries of these particles. Three different procedures were used to analyze the electron micrographs: (1) fuzzy-set based classification of the particles according to their rotational power spectra; (2) multivariate statistical analysis based on singular value decomposition; (3) circular harmonic analysis. Averages obtained from the three methods show unequivocally that the TF55-like complex presents a 9-fold symmetry.

引用

页码：152 / 155

页数：4

共 18 条

[1] GENERALIZED K NEAREST NEIGHBOR RULES [J].