EXTENT OF INCREASE IN INTRACELLULAR CALCIUM-CONCENTRATION IN ELECTRICALLY STIMULATED CHICK-EMBRYO HEART-CELLS DOES NOT DEPEND ON RESTING INTRACELLULAR CALCIUM-CONCENTRATION

被引：1

作者：

AHLEMEYER, B

WEINTRAUT, H

SCHONER, W

机构：

[1] JUSTUS LIEBIG UNIV GIESSEN,STRAHLENZENTRUM,D-35392 GIESSEN,GERMANY

[2] JUSTUS LIEBIG UNIV GIESSEN,INST BIOCHEM & ENDOKRINOL,D-35392 GIESSEN,GERMANY

来源：

CARDIOLOGY | 1993年 / 83卷 / 1-2期

关键词：

DIGITAL IMAGING MICROSCOPY; FURA-2; CULTURED CHICK EMBRYO HEART CELLS; EXCITATION CONTRACTION COUPLING; CA-2+](I);

D O I：

10.1159/000175948

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Using Fura-2 digital imaging microscopy, we found two cell populations in chick embryo heart with different resting intracellular calcium concentration ([Ca2+]i). Electrical stimulation of the cells led to the same increase in [Ca2+]i in both cell types, e.g. from 118 +/- 24 nM up to a peak of 243 +/- 67 nM (SD, n = 18) and from 44 +/- 15 nM up to a peak of 135 +/- 59 nM (SD, n = 13) in cells with high and low resting [Ca2+]i, respectively. All the cells showed the same rate of decline in [Ca2+]i. Due to synchronic stimulation and contraction, this method is suitable to measure [Ca2+]i transients at a high time resolution in individual cells.

引用

页码：55 / 60

页数：6

共 20 条

[1] CULTURED CHICK-EMBRYO HEART-CELLS RESPOND DIFFERENTLY TO OUABAIN AS MEASURED BY THE INCREASE IN THEIR INTRACELLULAR NA+ CONCENTRATION [J].