The tissue distribution of porcine 17 beta-estradiol dehydrogenase and its induction by progesterone

被引：28

作者：

Kaufmann, M ^{[1
]}

Carstensen, J ^{[1
]}

Husen, B ^{[1
]}

Adamski, J ^{[1
]}

机构：

[1] MAX PLANCK INST EXPTL ENDOCRINOL,D-30603 HANNOVER,GERMANY

来源：

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1995年 / 55卷 / 5-6期

关键词：

D O I：

10.1016/0960-0760(95)00203-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Porcine 17 beta-estradiol dehydrogenase (EDH) was recently purified and cloned. It catalyzes the NAD(+)-dependent oxidation of estradiol to estrone 360-fold more efficiently than the back reaction with NADPH. The 32 kDa EDH is cut from an 80 kDa primary translation product with a multidomain structure unknown for other hydroxysteroid dehydrogenases. The highest EDH activities and strongest immunoreactions are found in liver (hepatocytes) and kidney (proximal tubuli) followed by uterus (luminal and glandular epithelium), lung (bronchial epithelium). Progesterone treatment of ovariectomized gilts stimulates oxidative EDH activity in uterus, anterior pituitary, skeletal muscle (diaphragm) and kidney. Constitutive levels of EDH activity were seen in the adrenals, the lung and the liver.

引用

页码：535 / 539

页数：5

共 26 条

[1] PURIFICATION AND PROPERTIES OF ESTRADIOL 17-BETA-DEHYDROGENASE EXTRACTED FROM CYTOPLASMIC VESICLES OF PORCINE ENDOMETRIAL CELLS [J].

ADAMSKI, J ;

HUSEN, B ;

MARKS, F ;

JUNGBLUT, PW .

BIOCHEMICAL JOURNAL, 1992, 288 :375-381

[2] MOLECULAR-CLONING OF A NOVEL WIDELY EXPRESSED HUMAN 80 KDA 17-BETA-HYDROXYSTEROID DEHYDROGENASE-IV [J].

ADAMSKI, J ;

NORMAND, T ;

LEENDERS, F ;

MONTE, D ;

BEGUE, A ;

STEHELIN, D ;

JUNGBLUT, PW ;

DELAUNOIT, Y .

BIOCHEMICAL JOURNAL, 1995, 311 :437-443

[3] 17-BETA-HYDROXYSTEROID DEHYDROGENASE TYPE-2 - CHROMOSOMAL ASSIGNMENT AND PROGESTIN REGULATION OF GENE-EXPRESSION IN HUMAN ENDOMETRIUM [J].

CASEY, ML ;

MACDONALD, PC ;

ANDERSSON, S .

JOURNAL OF CLINICAL INVESTIGATION, 1994, 94 (05) :2135-2141

[4] STUDIES ON THE INVOLVEMENT OF LYSOSOMES IN ESTROGEN ACTION .3. THE DEHYDROGENATION OF ESTRADIOL TO ESTRONE BY PORCINE ENDOMETRIAL LYSOSOMES [J].

ENTENMANN, AH ;

SIERRALTA, W ;

JUNGBLUT, PW .

HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1980, 361 (06) :959-968

[5] ISOLATION AND SEQUENCING OF A COMPLEMENTARY DEOXYRIBONUCLEIC-ACID CLONE ENCODING HUMAN PLACENTAL 17-BETA-ESTRADIOL DEHYDROGENASE - IDENTIFICATION OF THE PUTATIVE COFACTOR BINDING-SITE [J].

GAST, MJ ;

SIMS, HF ;

MURDOCK, GL ;

GAST, PM ;

STRAUSS, AW .

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1989, 161 (06) :1726-1731

[6] MALE PSEUDOHERMAPHRODITISM CAUSED BY MUTATIONS OF TESTICULAR 17-BETA-HYDROXYSTEROID DEHYDROGENASE-3 [J].

GEISSLER, WM ;

DAVIS, DL ;

WU, L ;

BRADSHAW, KD ;

PATEL, S ;

MENDONCA, BB ;

ELLISTON, KO ;

WILSON, JD ;

RUSSELL, DW ;

ANDERSSON, S .

NATURE GENETICS, 1994, 7 (01) :34-39

[7]

GERSEVICH S, 1994, ENDOCRINOLOGY, V135, P1477

[8]

HILTUNEN JK, 1992, J BIOL CHEM, V267, P6646

[9]

HINZMANN RD, 1989, THESIS MAX PLANCK I

[10]

HUSEN B, 1994, CELL TISSUE RES, V278, P227, DOI 10.1007/BF00414164

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