INTEGRATION HOST FACTOR AFFECTS EXPRESSION OF 2 GENES AT THE CONJUGAL TRANSFER ORIGIN OF PLASMID R100

被引：24

作者：

DEMPSEY, WB ^{[1
]}

FEE, BE ^{[1
]}

机构：

[1] VET ADM MED CTR,DALLAS,TX 75216

来源：

MOLECULAR MICROBIOLOGY | 1990年 / 4卷 / 06期

关键词：

D O I：

10.1111/j.1365-2958.1990.tb00674.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Integration host factor (IHF) binds to two sites near the origin of transfer of the conjugative antibiotic resistance plasmid, R100. DNase I footprinting shows that one site is immediately adjacent to or/T and the gene X promoter, and another is adjacent to the traM promoter. A third site, known only from retardation gels, is near the traJ promoter. The relative promoter activities of genes X, traJ and traM are reduced in himA mutants (IHF), as measured by chloramphenicol‐resistance assays. Transcript analyses by Northern blots showed a reduction in size of the principal gene X and traJ transcripts in the absence of IHF. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：1019 / 1028

页数：10

共 36 条

[1]

BROSIUS J, 1987, METHOD ENZYMOL, V153, P54

[2]

BROSIUS J, 1982, J BIOL CHEM, V257, P9205

[3] PLASMID VECTORS FOR THE SELECTION OF PROMOTERS [J].