THE 31-KD MAJOR ALLERGEN, ALT A I1563, OF ALTERNARIA-ALTERNATA

被引：35

作者：

PARIS, S ^{[1
]}

DEBEAUPUIS, JP ^{[1
]}

PREVOST, MC ^{[1
]}

CASOTTO, M ^{[1
]}

LATGE, JP ^{[1
]}

机构：

[1] INST PASTEUR,CENT MICROSCOPIE ELECTRON STN,F-75724 PARIS 15,FRANCE

来源：

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY | 1991年 / 88卷 / 06期

关键词：

ALTERNARIA; ALLERGENS; GLYCOPROTEINS; PURIFICATION;

D O I：

10.1016/0091-6749(91)90247-L

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

A component of Alternaria extract, previously identified as the major allergen, Alt a I1563, was purified to homogeneity from Alternaria mycelium by means of acetone precipitation and ion-exchange chromatography. The homogeneity of Alt a I1563 was assessed by one single band after sodium dodecyl sulfate-polyacrylamide gel electrophoresis, by one single radiolabeled band after transfer to nitrocellulose, and by one single peak after size-exclusion chromatography by high-performance liquid chromatography. Alt a I1563 was isolated as a heat-stable acidic glycoprotein (carbohydrate content, 20%; pI, 4.0 to 4.5; 31 kd). The role of the carbohydrate moiety in the allergenicity is suggested. This major allergen is located in the cytoplasm of mycelium and spore.

引用

页码：902 / 908

页数：7

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