CHOLINESTERASE SOLUBILIZING FACTOR FROM CYTOPHAGA SP IS A PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C

被引:12
作者
JAGER, K [1 ]
STIEGER, S [1 ]
BRODBECK, U [1 ]
机构
[1] UNIV BERN,INST BIOCHEM & MOLEC BIOL,BUHLSTR 28,CH-3012 BERN,SWITZERLAND
关键词
GLYCOSYLPHOSPHATIDYLINOSITOL; GLYCOSYLPHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C; PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C; ACETYLCHOLINESTERASE; (CYTOPHAGA SP);
D O I
10.1016/0304-4165(91)90037-H
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In the culture supernatant of Cytophaga sp. we detected an enzyme that converted glycosylphosphatidyl-inositol-anchored acetylcholinesterase to the hydrophilic form. This enzyme had a cleavage specificity of a phospholipase C. It hydrolyzed phosphatidylinositol but did not act on phosphatidylcholine. On gel filtration the enzyme migrated with an apparent molecular mass of about 17 kDa. It displayed maximal activity between pH 6-6.5 and did not require cofactors for the expression of catalytic activity. Mercurials and zinc ions inhibited the enzyme and its activity also decreased with increasing ionic strength in the assay. With acetylcholinesterase as substrate optimal activity was obtained in pure micelles of Triton X-100, whereas in mixed micelles containing Triton X-100 and phosphatidylcholine the activity was reduced. The enzyme from Cytophaga sp. showed little activity towards acetylcholinesterase embedded in intact membranes where more than 1000-times higher concentrations of phosphatidylinositol-specific phospholipase C was necessary to solubilize acetylcholinesterase as compared to acetylcholinesterase in detergent micelles.
引用
收藏
页码:45 / 51
页数:7
相关论文
共 44 条
[1]   LEISHMANIA AND TRYPANOSOMA SURFACE GLYCOPROTEINS HAVE A COMMON GLYCOPHOSPHOLIPID MEMBRANE ANCHOR [J].
BORDIER, C ;
ETGES, RJ ;
WARD, J ;
TURNER, MJ ;
DEALMEIDA, MLC .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (16) :5988-5991
[3]  
BRODBECK U, 1981, MEMBRANE PROTEINS LA, P85
[4]   SYNTHESIS OF A HYDROLASE FOR THE MEMBRANE-FORM VARIANT SURFACE GLYCOPROTEIN IS REPRESSED DURING TRANSFORMATION OF TRYPANOSOMA-BRUCEI [J].
BULOW, R ;
OVERATH, P .
FEBS LETTERS, 1985, 187 (01) :105-110
[5]   A GLYCAN-PHOSPHATIDYLINOSITOL SPECIFIC PHOSPHOLIPASE-D IN HUMAN-SERUM [J].
DAVITZ, MA ;
HERELD, D ;
SHAK, S ;
KRAKOW, J ;
ENGLUND, PT ;
NUSSENZWEIG, V .
SCIENCE, 1987, 238 (4823) :81-84
[6]  
DAVITZ MAD, 1989, J BIOL CHEM, V261, P13760
[7]   A NEW AND RAPID COLORIMETRIC DETERMINATION OF ACETYLCHOLINESTERASE ACTIVITY [J].
ELLMAN, GL ;
COURTNEY, KD ;
ANDRES, V ;
FEATHERSTONE, RM .
BIOCHEMICAL PHARMACOLOGY, 1961, 7 (02) :88-&
[8]  
FERGUSON MAJ, 1985, J BIOL CHEM, V260, P4547
[9]  
FERGUSON MAJ, 1988, ANNU REV BIOCHEM, V57, P285
[10]   PURIFICATION OF A PHOSPHATIDYLINOSITOL-GLYCAN-SPECIFIC PHOSPHOLIPASE-C FROM LIVER PLASMA-MEMBRANES - A POSSIBLE TARGET OF INSULIN ACTION [J].
FOX, JA ;
SOLIZ, NM ;
SALTIEL, AR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (09) :2663-2667