MUTATIONAL ANALYSIS OF THE HUMAN P53 GENE IN MALIGNANT-MELANOMA

被引:91
作者
VOLKENANDT, M [1 ]
SCHLEGEL, U [1 ]
NANUS, DM [1 ]
ALBINO, AP [1 ]
机构
[1] MEM SLOAN KETTERING CANC CTR,1275 YORK AVE,NEW YORK,NY 10021
来源
PIGMENT CELL RESEARCH | 1991年 / 4卷 / 01期
关键词
TUMOR SUPPRESSOR GENE; TRANSFORMATION; HUMAN MELANOCYTES;
D O I
10.1111/j.1600-0749.1991.tb00311.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Nine metastatic melanoma cell lines and two melanocyte cell lines were analyzed for point mutations in highly conserved regions of the p53 gene. No mutations were detected in the two melanocytic cell lines and in eight melanoma cell lines. However, a C --> T transition at codon 248, resulting in a substitution of tryptophan for arginine, was found in one melanoma cell line. On immunohistochemical staining, only this cell line showed reactivity for mouse monoclonal antibody 1801, which is immunoreactive with human p53 protein. The original paraffin-embedded specimen from which this mutant cell line was established was obtained, and sequence analysis detected the identical mutation in the p53 gene as that seen in the derived cell line. This is the first report indicating point mutations in the p53 gene in malignant melanocytic tissues.
引用
收藏
页码:35 / 40
页数:6
相关论文
共 26 条
[1]  
ALBINO AP, 1989, ONCOGENE, V4, P1363
[2]   TRANSFORMING RAS GENES FROM HUMAN-MELANOMA - A MANIFESTATION OF TUMOR HETEROGENEITY [J].
ALBINO, AP ;
LESTRANGE, R ;
OLIFF, AI ;
FURTH, ME ;
OLD, LJ .
NATURE, 1984, 308 (5954) :69-72
[3]   CLASS-II HISTOCOMPATIBILITY ANTIGEN EXPRESSION IN HUMAN MELANOCYTES TRANSFORMED BY HARVEY MURINE SARCOMA-VIRUS (HA-MSV) AND KIRSTEN MSV RETROVIRUSES [J].
ALBINO, AP ;
HOUGHTON, AN ;
EISINGER, M ;
LEE, JS ;
KANTOR, RRS ;
OLIFF, AI ;
OLD, LJ .
JOURNAL OF EXPERIMENTAL MEDICINE, 1986, 164 (05) :1710-1722
[4]  
ALBINO AP, 1987, BASIC CLIN ASPECTS M, P3
[5]   ISOLATION OF HUMAN-P53-SPECIFIC MONOCLONAL-ANTIBODIES AND THEIR USE IN THE STUDIES OF HUMAN P53 EXPRESSION [J].
BANKS, L ;
MATLASHEWSKI, G ;
CRAWFORD, L .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1986, 159 (03) :529-534
[6]  
BARNEKOW A, 1987, CANCER RES, V47, P235
[7]  
BARTEK J, 1990, ONCOGENE, V5, P893
[8]   A VARIATION IN THE STRUCTURE OF THE PROTEIN-CODING REGION OF THE HUMAN-P53 GENE [J].
BUCHMAN, VL ;
CHUMAKOV, PM ;
NINKINA, NN ;
SAMARINA, OP ;
GEORGIEV, GP .
GENE, 1988, 70 (02) :245-252
[9]  
CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[10]   MUTATIONAL ANALYSIS OF HUMAN NRAS GENES IN MALIGNANT-MELANOMA - RAPID METHODS FOR OLIGONUCLEOTIDE HYBRIDIZATION AND MANUAL AND AUTOMATED DIRECT SEQUENCING OF PRODUCTS GENERATED BY THE POLYMERASE CHAIN-REACTION [J].
DICKER, AP ;
VOLKENANDT, M ;
ALBINO, AP .
GENES CHROMOSOMES & CANCER, 1990, 1 (04) :257-269