DIRECT DETERMINATION OF COTININE-N-GLUCURONIDE IN URINE USING THERMOSPRAY LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY

被引：16

作者：

BYRD, GD ^{[1
]}

UHRIG, MS ^{[1
]}

DEBETHIZY, JD ^{[1
]}

CALDWELL, WS ^{[1
]}

CROOKS, PA ^{[1
]}

RAVARD, A ^{[1
]}

RIGGS, RM ^{[1
]}

机构：

[1] UNIV KENTUCKY,COLL PHARM,LEXINGTON,KY 40536

来源：

BIOLOGICAL MASS SPECTROMETRY | 1994年 / 23卷 / 02期

关键词：

D O I：

10.1002/bms.1200230210

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A thermospray liquid chromatographic/mass spectrometric method has been developed for direct determination of cotinine-N-glucurinode in the urine of smokers. Quantification was performed using methyl-d(3)-cotinine-N-glucuronide as internal standard and monitoring the protonated aglycons. Using a simple preparation, urine samples from four smokers were analyzed and the results compared favorably with those from a previously reported method that quantifies aglycon release following beta-glucuronidase treatment. Amounts of cotinine-N-glucuronide found in urine from smokers ranged from less than 0.7 to 21 nmol ml(-1), indicating wide inter-individual variability in the metabolic production of this metabolite. Cotinine-N-glucuronide was found to be the second most abundant urinary nicotine metabolite. A similar method was developed for trans-3'-hydroxycotinine-N-glucuronide but this compound was not detected in smokers' urine.

引用

页码：103 / 107

页数：5

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