AMIDASE ACTIVE WHOLE CELLS OF CORYNEBACTERIUM-NITRILOPHILUS FOR AMMONIUM ACRYLATE PRODUCTION

被引：6

作者：

BRENNAN, MR ^{[1
]}

ARMITAGE, YC ^{[1
]}

MORTIMER, MG ^{[1
]}

HUGHES, J ^{[1
]}

RAMSDEN, M ^{[1
]}

机构：

[1] ALLIED COLLOIDS LTD,BRADFORD BD12 0JZ,W YORKSHIRE,ENGLAND

来源：

BIOTECHNOLOGY LETTERS | 1995年 / 17卷 / 05期

关键词：

D O I：

10.1007/BF00132020

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Corynebacterium nitrilophilus amidase was studied with a view to it's use in ammonium acrylate production. Treatment of whole cells with 1 M acrylamide and 2 M ammonium acrylate solutions significantly reduced amidase activity. Immobilized C. nitrilophilus cells were used in batch and continuous bioreactors. Operation of the continuous reactor was found to be the most convenient way of producing steady state conditions for the study of enzyme stability.

引用

页码：513 / 518

页数：6

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