SUCCINYL COA SYNTHETASE OF TOBACCO

被引:6
作者
BUSH, LP
机构
[1] Depailment of Agronomy, University of Kentucky, Lexington, Kentucky
关键词
D O I
10.1111/j.1399-3054.1969.tb09097.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The isouition and partial purification of succinyl CoA synthetase from Nicotiana tabacum is described. Enzynio activity had a pH optimum of pH 7.0 and apparent Km values for succinic acid. ATF and MgCl2. are 3.5 × 10−2 5.7 × 10−3 and 4.0 × 10−3 M, respectively. The euzyme is specific in requirement for ATP. Enzyme activity was low in seed, decreased during germination and seedling development, then increased to maximum in physiologically mature leaf tissue. Plauts with the apical apical meristem removed had less euzyme activity in leaves but more enzyme activity in the roots than plants with apical meristem intact. Most enzyme activity observed was associated with the soluble fractions of the cell. Copyright © 1969, Wiley Blackwell. All rights reserved
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页码:1097 / &
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