A NOVEL MEMBRANE FACTOR STIMULATES GUANINE-NUCLEOTIDE EXCHANGE-REACTION OF RAS PROTEINS

被引：107

作者：

WEST, M

KUNG, HF

KAMATA, T

机构：

[1] NCI,FREDERICK CANC RES FACIL,PROGRAM RESOURCES INC,BIOL CARCINOGENESIS & DEV PROGRAM,POB B,FREDERICK,MD 21701

[2] NCI,FREDERICK CANC RES FACIL,DIV CANC TREATMENT,BIOL RESPONSE MODIFIERS PROGRAM,BIOCHEM PHYSIOL,FREDERICK,MD 21701

来源：

FEBS LETTERS | 1990年 / 259卷 / 02期

关键词：

GDP/GTP exchange reaction; GTP-binding protein; Ras protein; Signal transduction;

D O I：

10.1016/0014-5793(90)80019-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A factor with a molecular weight of 100 kDa, which markedly enhanced the guanine nucleotide exchange reaction of ras p21 proteins, was partially purified from bovine brain tissues. The factor was predominantly associated with the plasma membrane. When the partially purified factor and excess cold GTP were added to [3H]GDP . ̄Gly12 p21 or Vall2 p21 in the presence of 2 mM MgCl2, the nucleotide exchange rate was stimulated up to 25-fold. The stimulation of the p21-nucleotide exchange reaction by the factor was completely blocked by the Y13-259 ras-neutralizing antibody. Taken together, these results suggest that the factor may control the rate limiting GDP/GTP exchange step in recycling of p21 in ras-mediated signal transduction. © 1990.

引用

页码：245 / 248

页数：4

共 18 条

[1] RAS GENES [J].